声明
List of Abbreviations
Chapter 1 INTRODUCTION
1.1 Importance of cotton
1.2 Global extent of salinity and challenges for cotton production
1.3 Cotton response to salt stress
1.4 Salt tolerance mechanisms of plant
1.4.1 Ion homeostasis and sequestration
1.4.2 Osmotic adjustment
1.4.3 Scavenging of reactive oxygen species (ROS)
1.4.4 Modulation of photosynthesis
1.5 Management approach for salt tolerance through nitrogen nutrition
1.6 Nitrogen supplemented salt tolerance mechanism
1.7 Nitrogen supplemented salt tolerance of cotton
1.8 Aims and objectives
Chapter 2 SCREENING COTTON GENOTYPES FOR SALT TOLERANCE
2.1 Introduction
2.2 Materials and methods
2.2.1 Plant materials, growth environments, and treatments
2.2.2 Measurement of growth traits
2.2.3 Determination of leaf relative water content
2.2.4 Estimation of gas exchange parameters
2.2.5 Assay of lipid peroxidation
2.2.6 Tissue elemental inorganic ion analysis
2.2.7 Salt tolerance evaluation
2.2.8 Statistical analysis
2.3 Results
2.3.1 Determination of optimum salt concentration
2.3.2 Genotypic variation under salt stress
2.3.3 Correlation analysis under salt stress
2.3.4 Determining reliable salt tolerance traits
2.3.5 Cluster heat map analysis
2.4 Discussion
2.5 Conclusions
Chapter 3 NITROGEN MEDIATED SALT STRESS ALLEVIATION
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant materials, growth environments, and experimental treatments
3.2.2 Estimation of growth parameters
3.2.3 Biomass determination
3.2.4 Determination of photosynthetic pigments
3.2.5 Measurement of gas exchange attributes
3.2.6 Determination of oxidative stress markers
3.2.7 Determination of soluble sugar, soluble protein, and free amino acid
3.2.8 Determination of leaf relative water content
3.2.9 Assay of antioxidant enzymes
3.2.10 Statistical analysis
3.3 Results
3.3.1 Nitrogen supplementation increased growth and biomass accumulation
3.3.2 Nitrogen addition improves pigment synthesis
3.3.3 Influence of nitrogen on gas exchange attributes in response to salinity stress
3.3.4 Nitrogen induces osmolyte accumulation under salt stress
3.3.5 Application of nitrogen improves plant water status
3.3.6 Application of nitrogen mitigates oxidative damage
3.3.7 Nitrogen up-regulates the antioxidant activity under salt stress
3.3.8 Cluster heat map analysis
3.4 Discussion
3.5 Conclusions
Chapter 4 EFFECT OF NITROGEN AND SALT ON GROWTH AND YIELD OF COTTON
4.1 Introduction
4.2 Materials and methods
4.2.1 Plant materials, growth conditions, and treatments
4.2.2 Measurement of growth traits and biomass accumulation
4.2.3 Determination of root morphology
4.2.4 Determination of photosynthetic pigments
4.2.5 Determination of leaf gas exchange attributes
4.2.6 Determination of nitrogen content
4.2.7 Determination of yield attributes
4.2.8 Statistical analysis
4.3 Results
4.3.1 Positive effect of nitrogen on cotton growth traits
4.3.2 Nitrogen improves biomass accumulation under salt stress
4.3.3 Nitrogen improves the root morphology under salt stress
4.3.4 Nitrogen enhances photosynthetic pigment biosynthesis
4.3.5 Nitrogen improves leaf gas exchange attributes
4.3.6 Exogenous nitrogen supplementation increases the nitrogen content
4.3.7 Nitrogen progressively enhances the yield attributes of cotton
4.3.8 Radar plot analysis
4.4 Discussion
4.5 Conclusions
Chapter 5 DISCUSSION AND CONCLUSIONS
5.1 Discussion
5.2 Conclusions
5.3 Future perspective
参考文献
致谢
CURRICULUM VITAE
APPENDICES
中国农业科学院;
