声明
Chapter I Introduction
1.1 Tomato
1.2 Whitefly
1.3Whitefly and tomato
1.4Whitefly feeding behaviour
1.5Nature of damage of Whiteflies
1.6Virus transmission
1.7Whitefly control
1.8Entomopathogenic Fungi
1.9How Do Entomopathogenic Fungi (EPFs) Behave?
1.10Beauveria bassiana
1.10.1Morphology, cultural characteristics and molecular characterization of Beauveria bassiana
1.10.2Life cycle of Beauveria bassiana
1.10.3Factors responsible for germination of conidia of Beauveria bassiana
1.10.4Pathogenicity of Beauveria bassiana
1.10.5Beauveria bassiana as a Biocontrol agent
1.10.6Agricultural importance of Beauveria bassiana
1.11Verticillium lecanii(Lecanicillium lecanii)
1.11.1Insecticidal potential of V. lecanii
1.12Defense Mechanisms of Plants to Insect Pests
1.13Host Plant Defenses against Insects
1.14Morphological features for physical defense
1.15 Elicitors
1.16Use of elicitors in defense mechanism
1.17Classification of elicitors
1.17.1General elicitors
1.17.2Specific elicitors (Effectors)
1.18Secondary metabolites for chemical defense of plants
1.19Plant defensive proteins
1.20Herbivore induced plant volatiles (HIPVs)
1.21Defense elicitors (insect oral secretion)
1.22Role of phytohormones in induced resistance in plants
Chapter 2 In Vitro Pathogenicity of the fungi;Beauveria bassiana and Lecanicillium lecaniiat different temperatures against the Whitefly,Bemisia tabaci(Genn.) (Hemiptera: Aleyrodidae)
2.1 Introduction
2.2Materials and Methods
2.2.1Whitefly stock culture
2.2.3Effect of temperature on fungus virulence against Bemisia tabaci
2.2.4 Fungal isolates
2.2.5Conidial suspensions
2.2.6Fungal filtrate
2.2.7Pathogenicity Bioassays
2.2.8Data Analysis
2.3 Results
2.3.1Conidial Virulence
2.3.2Filtrate Pathogenicity
2.3.3Binary Combinations Entomopathogenicity
2.4 Discussion
Chapter 3 Effect of partially purified protein derived from Beauveria bassiana on expression of defense related genes in tomato against whitefly
3.1 Introduction
3.2Material Method
3.2.1Plant culture and insect rearing
3.2.2Fungal culture
3.2.3Crude protein preparation and partial purification
3.2.4Protein activity bioassay against B. tabaci
3.2.5RNA extraction and cDNA synthesis
3.2.6Reverse transcription-quantitative PCR (RT-qPCR)
3.2.7Statistical analysis
3.3 Result
3.3.1Purification of the Beauveria bassiana ARSEF 2860 crude protein
3.3.2B. bassiana protein effect on B. tabacis urvival
3.3.3Effect of B. bassiana derivative protein on fecundity B. tabaci
3.3.4Plant defense gene expression level in response to B. bassiana protein
3.4 Discussion
3.5 Conclusions
Chapter 4 Protein elicitor PeBA1enhanced resistance against whitefly Bemisia tabaci(Genn.) (Hemiptera: Aleyrodidae) in tomato (Solanum lycopersicum)
4.1 Introduction
4.2Material methods
4.2.1Insects and host plants
4.2.2Protein purification
4.2.3Characterization of protein
4.2.4Isolation of RNA and cDNA synthesis
4.2.5Reverse Transcription–Quantitative PCR (RT-qPCR)
4.2.6Statistical analysis
4.3 Results
4.3.1Expression, purification and evaluation of PeBA1 elicitor protein
4.3.2Effect of PeBA1 Elicitor on the Fecundity Rate
4.3.3Expression of Plant Defense associated genes
Discussion
参考文献
致谢
Resume
中国农业科学院;
