声明
Table of Contents
Abstract
摘要
Abbreviations
Chapter 1 Background，Literature Review and Objectives
1 Aquaculture production-overall background
2 The common carp(Cyprinus carpio L．)
2．1 Production in China
2．2 Genetic characteristics and improvement
3 Applied genetically improved species in aquaculture
3．1 Molecular bases for desirable traits in aquaculture
3．2 Molecular breeding and marker-assisted selection
4 The use of genome-wide technology in selective breeding
5 Growth performance as a basis of genetic selection in Fish
6 Sex control in aquaculture
6．1 Sexual differentiation in fish
6．2 Sex determination in aquaculture
7 Hypothesis and aims of the study
8 Objectives of the Study
Chapter 2 Genome Scan for Candidate Growth Performance Loci Exploring in a New Fast Growing Common Carp Strain(Cyprinus carpio L．)
Introduction
1 Materials and methods
1．1 Experimental material and sampling
1．2 Ethical statement
1．3 DNA extraction and 2b-RAD sequencing
1．4 2b-RAD raw data processing
1．5 Genome wide analysis and candidate SNP identification
1．6 Statistical data analysis
2 Results
2．1 Growth-related traits evaluation
2．2 2b-RAD raw data processing
2．3 Genomic marker associated with growth performance parameters
3 Discussion
Conclusion
Chapter 3 Genome Scan for Candidate Sex Determination Loci Exploring in a New Fast Growing Common Carp Strain (Cypriuns carpio L．)
Introduction
1 Materials and methods
1．1 Experimental material and sampling
1．2 Ethical statement
1．3 DNA extraction and purification
1．4 2B-RAD sequencing and genotyping
1．5 GWAS and threshold levels analysis
1．6 Sequence-based on different coverage depth analysis
1．7 Polymerase chain reaction analysis
1．8 Statistical data analysis
2 Results
2．1 Sex effect on growth performance of Hnanghe carp new strain
2．2 Different SNPs found between male and female
2．3 Different methods to explore the sex-related molecular markers
2．4 ADAM10 selected annotation and character in Huanghe new strain
2．5 Analysis of the inheritance
3 Discussion
Conclusion
General conclusions
References
Appendix
Acknowledgements
Publications，Seminars and Symposia Attended
南京农业大学;
