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首页> 外文学位 >Physical map between marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that contains the NIP gene.
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Physical map between marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that contains the NIP gene.

机译：梅花苜蓿连锁群1上包含NIP基因的标记8O7和146O17之间的物理图谱。

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摘要

The Medicago truncatula NIP gene is located on M. truncatula Linkage Group 1. Informative recombinants showed crossovers that localize the NIP gene between markers 146O17 and 23C16D. Marker 164N9 co-segregates with the NIP gene, and the location of marker 164N9 is between markers 146O17 and 23C16D. Based upon data from the Medicago genome sequencing project, a subset of the model legume Medicago truncatula bacterial artificial chromosomes (BACs) were used to create a physical map on the DNA in this genetic internal. BACs near the potential NIP gene location near marker 164N9 were identified, and used in experiments to predict the physical map by a BAC-by-BAC strategy. Using marker 164N9 as a center point, and chromosome walking outward, the physical map toward markers 146O17 and 23C16D was built. The chromosome walk consisted of a virtual walk, made with existing sequence of BACs from the Medicago genome project, hybridizations to filters containing BAC DNA, and PCR reactions to confirm that predicted overlapping BACs contained DNA that yielded similar PCR products. In addition, the primers which are made for physical mapping via PCR could be good genetic markers helpful in discovering the location of the NIP gene. As a result of efforts repotted here, gap in physical map between marker 164N9 and 146O17 was closed.
机译：梅花苜蓿NIP基因位于梅花苜蓿连锁群1上。信息性重组子显示出将NIP基因定位在标记146O17和23C16D之间的交换。标记164N9与NIP基因共分离，并且标记164N9的位置在标记146O17和23C16D之间。根据来自Medicago基因组测序项目的数据，模型豆科植物苜蓿截骨bacterial细菌人工染色体（BAC）的一个子集用于在此遗传内部的DNA上创建物理图谱。确定了靠近标记164N9的潜在NIP基因位置附近的BAC，并在实验中通过BAC-by-BAC策略预测了物理图谱。使用标记164N9作为中心点，染色体向外行走，建立了向标记146O17和23C16D的物理图。染色体行走由虚拟行走组成，该行走由来自Medicago基因组计划的现有BAC序列，与包含BAC DNA的滤膜杂交以及进行PCR反应以确认预测的重叠BAC包含可产生相似PCR产物的DNA组成。此外，通过PCR进行物理作图的引物可能是良好的遗传标记，有助于发现NIP基因的位置。由于在此进行了努力，因此标记164N9和146O17之间的物理图谱之间的差距被消除。

著录项

  • 作者

    Lee, Yi-Ching.;

  • 作者单位

    University of North Texas.;

  • 授予单位 University of North Texas.;
  • 学科 Biology Botany.;Biology Genetics.
  • 学位 M.S.
  • 年度 2007
  • 页码 133 p.
  • 总页数 133
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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