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Functional studies of two rice genes related to signal transduction of defense responses.

机译：两个水稻基因的功能研究与防御反应的信号转导有关。

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摘要

Biotic stress is one of the most serious constraints on rice productivity. Strategy adopting regulators in signal transduction of systemic acquired resistance for conferring long-lasting disease resistance against broad spectrum of pathogens become highly favorable. To achieve this, signal transduction of disease resistance should be well characterized.OsGPBP1 is a putative G-protein binding protein and interacts with a member of the YchF G-protein subfamily that has not been thoroughly studied in plants, while OsRHC1 is a novel RING zinc finger protein harboring multiple transmembrane domains at the N-half and a unique RING-HC domain at the C terminus. Both of them were induced in the bacterial blight resistant near isogenic rice line upon wounding. Gain-of-function tests in transgenic Arabidopsis thaliana showed that their ectopic expressions are able to trigger the expression of both defense marker genes mediated either by SA- or JA/ET-pathways and led to increased resistance toward the pathogen Pseudomonas syringae pv. tomato DC3000 and both of the two clones seemed to rely on NPR1 (disease resistance key regulator) for function. Furthermore, over-expressions of the two clones in its native system are also able to activate rice defense marker genes.Suppression subtractive hybridization experiment, using RNA samples from a pair of near-isogenic rice lines either containing the R gene Xa14 (CBB14) or its susceptible recurrent parent (SN1033), were previously performed in our laboratory. Two gene candidates ( OsGPBP1 and OsRHC1) probably encoding two novel types of signal transduction components related to disease resistance are chosen for further analysis.
机译：生物胁迫是对水稻生产力的最严重限制之一。在全身性获得性抗性的信号转导中采用调节剂以赋予对多种病原体的持久疾病抗性的策略变得非常有利。为此，应很好地表征抗病性的信号转导。OsGPBP1是一种推定的G蛋白结合蛋白，可与尚未在植物中进行深入研究的YchF G蛋白亚家族成员相互作用，而OsRHC1是一种新型RING。锌指蛋白在N-半部带有多个跨膜结构域，在C端具有唯一的RING-HC结构域。两者均在受伤时在等基因水稻系附近的抗白叶枯病中被诱导。在转基因拟南芥中的功能获得测试表明，它们的异位表达能够触发由SA途径或JA / ET途径介导的两种防御标记基因的表达，并导致对病原体丁香假单胞菌pv的抗性增加。番茄DC3000和两个克隆似乎都依赖NPR1（抗病性关键调节剂）发挥功能。此外，两个克隆在其天然系统中的过表达也能够激活水稻防御标记基因。使用来自一对具有R基因Xa14（CBB14）或其易感的轮回亲本（SN1033），以前在我们的实验室中进行过。选择了可能编码与疾病抗性有关的两种新型信号转导成分的两个基因候选物（OsGPBP1和OsRHC1）进行进一步分析。

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  • 作者

    Cheung, Ming Yan.;

  • 作者单位

    The Chinese University of Hong Kong (Hong Kong).;

  • 授予单位 The Chinese University of Hong Kong (Hong Kong).;
  • 学科 Biology Molecular.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 205 p.
  • 总页数 205
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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