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首页> 外文学位 >Microfabricated high-performance liquid chromatography (HPLC) system with closed-loop flow control.
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Microfabricated high-performance liquid chromatography (HPLC) system with closed-loop flow control.

机译：具有闭环流量控制功能的微型高效液相色谱（HPLC）系统。

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摘要

This thesis presents the development of a microfabricated high-performance liquid chromatography (HPLC) system. The design, fabrication, and characterization of individual HPLC components such as high-pressure pumps, mixers, flow sensors, composition sensors, separation columns, filters, and detectors is presented. These individual components were then integrated to create robust, feedback-driven separation systems capable of performing gradient, reverse-phase, nanoscale HPLC. Two separate separation systems were created. The first integrated system was a microfluidic device for HPLC tandem mass spectrometry (HPLC-MS/MS) designed for proteomic applications. The second system was a portable HPLC conductivity detection (HPLC-CD) system designed for point-of-care applications such as biodetection. Both systems demonstrated good performance and repeatability. The performance of these systems is largely attributable to the development of HPLC-compatible sensors that could provide precise control over the elution profiles. These microfluidic closed-loop flow control systems represent an important advancement in the microfluidics field, where open-loop flow control is universally used, and risks becoming inadequate with the increasing complexity of microfluidic systems.
机译：本文提出了一种微型高效液相色谱（HPLC）系统的开发。介绍了各个HPLC组件（如高压泵，混合器，流量传感器，成分传感器，分离柱，过滤器和检测器）的设计，制造和特性。然后将这些单独的组件集成在一起，以创建能够执行梯度，反相，纳米级HPLC的强大的，反馈驱动的分离系统。创建了两个单独的分离系统。第一个集成系统是用于蛋白质组学应用的HPLC串联质谱（HPLC-MS / MS）的微流控设备。第二个系统是便携式HPLC电导率检测（HPLC-CD）系统，设计用于即时检测应用，例如生物检测。两种系统都表现出良好的性能和可重复性。这些系统的性能很大程度上归功于HPLC兼容传感器的开发，该传感器可提供对洗脱曲线的精确控制。这些微流体闭环流量控制系统代表了微流体领域的重要进步，在微流体领域中，普遍使用开环流量控制，并且随着微流体系统复杂性的增加，风险会变得不足。

著录项

  • 作者

    Shih, Jason.;

  • 作者单位

    California Institute of Technology.;

  • 授予单位 California Institute of Technology.;
  • 学科 Electrical engineering.;Analytical chemistry.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 178 p.
  • 总页数 178
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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