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A microfluidic device for continuous capture and concentration of pathogens from water.

机译：一种用于持续捕获和浓缩水中病原体的微流控设备。

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摘要

A microfluidic device, based on electrophoretic transport and electrostatic trapping of charged particles, has been developed for continuous capture and concentration of microorganisms from water. A generic design, utilizing mobility and zeta potential measurements of various microorganisms exposed to different environmental conditions and physiological states, was employed. Water and buffer samples at pH values ranging from 5.2--7.0 were seeded with bacteria (E. coli, Salmonella, and Pseudomonas) and viruses (MS-2 and Echovirus). Negative control and capture experiments were performed simultaneously using two identical devices. Both culture based methods and real-time PCR analysis were utilized to characterize the capture efficiency as a function of time, flowrate, and applied electric field. Based on differences between the capture and negative control data, capture efficiencies of 90% to 99% are reported for E. coli, Salmonella, Pseudomonas, and MS-2, while the capture efficiency for Echovirus was around 75%. Overall, the device exhibits 16.67 fold sample volume reduction within an hour at 6 mL/hr. This results in a concentration factor of 15 at 90% capture efficiency. Direct quantification of capture on the anode of the prototype microfluidic device was also performed by particle tracking using fluorescent microscopy. Based on image processing, the capture data at different locations on the electrode surface is quantified as a function of the wall shear stress at these locations, which is calculated using CFD simulations. Finally, the Faradaic processes in the microchannel due to electrochemical reactions are studied to predict the amount of electrophoresis in the system.; Scaling of the device to sample 5 L/hr can be achieved by stacking 835 identical microchannels. Power and wetted volume for the prototype and scaled devices are presented. The device can thus function either as a filtration unit or as a sample concentrator to enable the application of real-time detection sensor technologies. The ability to continuously sample water without chemical additives facilitates the use of this device in drinking water distribution systems. This work constitutes the first step in our development of a continuous, microbial capture and concentration system from large volumes of potable water.
机译：已经开发出一种基于电泳传输和带电粒子静电捕获的微流体装置，用于连续捕获和浓缩水中的微生物。采用通用设计，利用暴露于不同环境条件和生理状态的各种微生物的迁移率和ζ电位测量。将pH值在5.2--7.0范围内的水和缓冲液样品接种细菌（大肠杆菌，沙门氏菌和假单胞菌）和病毒（MS-2和Echovirus）。使用两个相同的设备同时进行阴性对照和捕获实验。利用基于培养的方法和实时PCR分析来表征捕获效率随时间，流速和施加电场的变化。根据捕获和阴性对照数据之间的差异，据报道大肠杆菌，沙门氏菌，假单胞菌和MS-2的捕获效率为90％至99％，而回声病毒的捕获效率约为75％。总体而言，该设备在6 mL / hr的时间内在一小时内样品体积减少了16.67倍。在90％的捕获效率下，浓缩系数为15。通过使用荧光显微镜的颗粒跟踪，还可以对原型微流体装置阳极上的捕获量进行直接定量。基于图像处理，将电极表面上不同位置处的捕获数据量化为这些位置处壁剪切应力的函数，这是使用CFD模拟计算得出的。最后，研究了由于电化学反应导致的微通道中的法拉第过程，以预测系统中的电泳量。通过堆叠835个相同的微通道，可以将设备缩放至5 L / hr。展示了原型和定标设备的功率和湿润体积。因此，该设备既可以充当过滤单元，也可以充当样品浓缩器，以实现实时检测传感器技术的应用。无需化学添加剂即可连续采样水的功能有助于在饮用水分配系统中使用该设备。这项工作是我们从大量饮用水中开发连续的微生物捕获和浓缩系统的第一步。

著录项

  • 作者

    Balasubramanian, Ashwin Kumar.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Engineering Mechanical.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 机械、仪表工业;
  • 关键词

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