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首页> 外文学位 >Fixed-time insemination of porcine luteinizing hormone-treated superovulated beef cows and the resynchronization of beef cows for fixed-time embryo transfer.
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Fixed-time insemination of porcine luteinizing hormone-treated superovulated beef cows and the resynchronization of beef cows for fixed-time embryo transfer.

机译：固定时间授精猪黄体化激素处理的超排卵肉牛，以及固定时间胚胎移植的肉牛再同步。

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摘要

Two trials were conducted to compare the effectiveness of fixed-time artificial insemination (AI) to AI based upon visual detection of estrus following superstimulation of donor beef cows. In Trial 1, multiparous beef cows (n = 31) were randomly allotted to one of three treatments following superstimulation and removal of an intravaginal progesterone insert (CIDR). Cows in the Control group were inseminated at 12 and 24 h after onset of estrus. Cows in the Estradiol group were injected with estradiol-17β (1 mg, im) at 12 h and inseminated at 24 and 36 h after CIDR removal. Cows in the pLH36 group were injected with porcine LH (Lutropin, 12.5 mg, im) at 24 h and inseminated at 36 and 48 h after CIDR removal. Mean numbers of viable embryos were 7.8, 3.6 and 9.6 for Control, Estradiol and pLH36 treatment groups, respectively (P > 0.10). In Trial 2, multiparous beef cows (n = 22) were randomly allotted to one of three treatments following superstimulation and removal of a CIDR. Sixteen of the cows were superstimulated a second time approximately 50 days later and allotted to one of the two treatments that differed from the initial treatment group. Cows in the Control group were inseminated at 12 and 24 h after onset of estrus. Cows in the two pLH groups were injected with porcine LH (Lutropin, 12.5 mg, im) at 24 h after CIDR removal and were inseminated with either one unit of semen at 36 and 48 h (pLH36) or with two units of semen at 48 h (pLH48) after CIDR removal. Mean numbers of viable embryos were 3.0, 6.4 and 3.8 for Control, pLH36 and pLH48 treatment groups, respectively (P > 0.10). These data indicate that administration of pLH can facilitate use of fixed-time AI in superovulated beef cows without sacrificing embryo production.;The second study evaluated the efficacy of resynchronizing beef cow recipients using CIDR devices for only 7 or 14 d. Recipient cows received CIDRs either on the day of transfer (n = 88) or 7 d post-transfer (n = 230). All CIDRs were removed on d 21 and cows were observed for estrus between d 22 and 24. Cows that displayed estrus were ultrasounded on d 30, those cows not pregnant that possessed a CL had an embryo transferred that day. Cows were later examined for pregnancies approximately 23 to 30 d later. There were no differences in pregnancy rates between cows with 7 or 14 d CIDRs and therefore data were combined. Pregnancy rates at two different ranches indicate that beef cow recipients can be successfully resynchronized by insertion of a CIDR without compromising pregnancy rates of transferred embryos. At Center Ranch the pregnancy rate for the first transfer was 57% while the resynchronized group that received the second transfer had a pregnancy rate of 55%. At Mound Creek Ranch the first transfer of embryos produced 59% pregnancy rates while the second transfer had a pregnancy rate of 71%. No significant differences (P > 0.05) were observed between the pregnancy rates of the initial transfer and those of the resynchronized transfer using only CIDRs, indicating that resynchronization using CIDRs can be used without reducing pregnancy rates.
机译：进行了两项试验，根据肉眼对供体肉牛超刺激后发情的视觉检测，比较了固定时间人工授精（AI）与AI的有效性。在试验1中，在过度刺激和取出阴道内孕酮插入物（CIDR）之后，将多头肉牛（n = 31）随机分配给三种处理之一。对照组在发情开始后12和24 h进行授精。雌二醇组的母牛在12 h注射雌二醇17β（1 mg，im），并在CIDR去除后24和36 h授精。 pLH36组的母牛在24 h注射猪LH（Lutropin，12.5 mg，im），并在CIDR去除后36和48 h授精。对照组，雌二醇和pLH36治疗组的活胚平均数分别为7.8、3.6和9.6（P> 0.10）。在试验2中，在刺激和去除CIDR之后，将多头肉牛（n = 22）随机分配给三种处理之一。大约50天后，第二次对16头牛进行超刺激，并分配给与初始治疗组不同的两种治疗方法之一。对照组在发情开始后12和24 h进行授精。两组pLH组的母牛在去除CIDR后24 h注射猪LH（Lutropin，12.5 mg，im），并在36和48 h用一单位精液（pLH36）或在48 h用两单位精液进行授精。去除CIDR后的h（pLH48）。对照组，pLH36和pLH48治疗组的活胚平均数分别为3.0、6.4和3.8（P> 0.10）。这些数据表明，pLH的施用可以促进在超排卵的肉牛中使用固定时间的AI，而不会牺牲胚胎的产生。；第二项研究评估了使用CIDR设备使肉牛受体重新同步仅7或14天的功效。接受奶牛在转移当天（n = 88）或转移后7天（n = 230）接受CIDR。在第21天删除所有CIDR，并在22到24天之间观察到母牛的发情。在第30 d对显示发情的母牛进行超声检查，那些没有CL的未怀孕的母牛当天移植了胚胎。约23至30 d之后，对母牛进行了妊娠检查。 CIDR 7或14 d的母牛的妊娠率没有差异，因此将数据合并。两个不同牧场的怀孕率表明，通过插入CIDR可以成功使肉牛接受者重新同步，而不会影响转移胚胎的怀孕率。在中心牧场，第一次转移的妊娠率为57％，而接受第二次转移的再同步组的妊娠率为55％。在蒙德克里克牧场（Mound Creek Ranch），第一次胚胎移植的妊娠率为59％，而第二次胚胎的妊娠率为71％。初始转移的妊娠率与仅使用CIDR的重新同步转移的妊娠率之间没有观察到显着差异（P> 0.05），这表明可以使用CIDR进行重新同步而不会降低妊娠率。

著录项

  • 作者

    Nelson, John Stephen.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Biology Animal Physiology.;Agriculture Animal Culture and Nutrition.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 86 p.
  • 总页数 86
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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