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Regeneration of spent dialysate using a membrane bioreactor.

机译：使用膜生物反应器再生废透析液。

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A membrane bioreactor (MBR) system using Sporosarcina pasteurii for the removal of urea from spent dialysate was investigated. Work entailed the identification of growth conditions for S. pasteurii in batch, continuous, and MBR formats to understand nutritional requirements, growth parameters, and ultimately, clearance of urea. During the preliminary studies, a method for growing S. pasteurii in spent dialysate was developed. S. pasteurii lag phase decreased from 150h to a few hours when 1% (w/w) yeast extract and 10% of acclimated inoculum source were used. Acclimated S. pasteurii used as working stock has a lifetime of up to 15h at 30°C. The kinetic parameters of S. pasteurii in spent dialysate were studied using a batch reactor and a chemostat. During the batch reactor tests, S. pasteurii growth could be modeled with Blackman's equation for glucose concentration lower than 2g/L. The maximum specific growth rate for S. pasteurii was 0.113h-1. When grown in chemostat, S. pasteurii growth exceeded the predicted maximum dilution rate based on preliminary batch values.; Urea removal as high as 90% could be achieved during the membrane bioreactor studies. Dilution rates as high as approximately 20 times the expected critical dilution rate could be realized, and large solid retention times did not affect membrane flux. The polysulfone membrane with a molecular weight cut off of 10,000 produced a flux as high as 20L/m2h. Glucose consumption was negligible during the MBR runs. Finally, a preliminary economic analysis of a MBR system used to degrade urea was completed. The analysis showed a return on investment of 115% when a dialysis clinic uses regenerated spent dialysate with S. pasteurii in a MBR system.
机译：研究了一种膜生物反应器（MBR）系统，该系统使用巴斯德孢子菌从废透析液中去除尿素。工作需要分批，连续和MBR形式确定巴氏链球菌的生长条件，以了解营养需求，生长参数以及最终清除尿素。在初步研究中，开发了一种在废透析液中生长巴氏酵母的方法。当使用1％（w / w）酵母提取物和10％的适应接种源时，巴氏酵母的滞后期从150h减少到几个小时。用作工作原料的驯化巴氏酵母在30°C下的使用寿命长达15h。使用间歇反应器和化学恒温器研究巴氏酵母在废透析液中的动力学参数。在间歇反应器测试期间，巴氏酵母的生长可以用布莱克曼方程式模拟葡萄糖浓度低于2g / L。巴氏酵母的最大比生长速率为0.113h-1。当在恒化器中生长时，巴氏酵母的生长超过基于初步批次值的预测最大稀释率。在膜生物反应器研究期间，尿素去除率可高达90％。可以实现高达预期临界稀释率约20倍的稀释率，并且较大的固体保留时间不会影响膜通量。截留分子量为10,000的聚砜膜产生的通量高达20L / m2h。 MBR运行期间的葡萄糖消耗微不足道。最后，完成了用于降解尿素的MBR系统的初步经济分析。分析显示，当透析诊所在MBR系统中使用再生的巴斯德氏菌用过的透析液时，投资回报率为115％。

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