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Chemical sensor development leading to an alternative glucose detection method.

机译：化学传感器的发展导致了一种替代性的葡萄糖检测方法。

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摘要

The development of a viable sensing platform for the purpose of small molecule detection was explored on two different platforms: a Metal Oxide Semiconductor Field Effect Transistor (MOSFET) and an Interdigitated Electrode (IDE). A standard operating procedure (SOP) for the reliable fabrication of gold gated MOSFET platforms and IDE platforms was created via standard cleanroom techniques.; The n-MOSFET platform (p-channel device) drain current ( IDS) shifts due to the binding of a self assembled monolayer (SAM) onto the Au-gated FET. The IDS directly correlates to the (VG - VTH) 2. Binding of an electron donating p-diethyamino phenyl isocyanide SAM onto the gate results in an positive shifted IDS/decreased VTH, while an electron withdrawing species, 1,4-phenyl diisocyanide SAM exhibits a negative IDS/increased VTH. These changes in VTH shift corresponds to an electron donating species acting as a positive gate bias into the gold gate while electron acceptors acts as a negative gate bias.; Binding studies show thiophene 3-boronic acid (TBA) readily binds glucose with a binding constant of Keq = 42M-1, an order of magnitude larger than reported literature values for phenyl boronic acid. Chemical binding of 1,2-diol to poly-TBA was shown by fluorescent emission spectroscopy. Crystal structures of TBA bound to ethylene glycol and pinnacol confirm 1,2-diol binding. An IDE platform was functionalized with thiophene 3-boronic acid (TBA) polymer via electrochemical deposition for direct detection of 1,2-diols. Conduction measurements were made upon exposure to 1,2-diol functional groups such as glucose. Buffered (pH 7.4) glucose solutions from 1--10 mM exhibited increases in the current across the poly-TBA, and attributed to glucose binding.; We have demonstrated by chemical, optical, and electrochemical methods that 1,2-diols such as glucose directly binds to poly-TBA and is an alternative to traditional indirect glucose detection methods.
机译：在两个不同的平台上探索了用于小分子检测的可行传感平台的开发：金属氧化物半导体场效应晶体管（MOSFET）和叉指电极（IDE）。通过标准洁净室技术，建立了用于可靠制造金门控MOSFET平台和IDE平台的标准操作程序（SOP）。由于将自组装单层（SAM）绑定到Au门控FET上，n-MOSFET平台（p沟道器件）的漏极电流（IDS）移动。 IDS与（VG-VTH）2直接相关。将给电子的对二乙氨基苯基异氰酸酯SAM结合到栅极上会导致IDS正向偏移/ VTH降低，而吸电子物质1,4-苯基二异氰酸酯SAM呈现负IDS /增加的VTH。 VTH偏移的这些变化对应于给电子物质，作为进入金门的正门偏压，而电子受体作为负门偏压。结合研究表明，噻吩3-硼酸（TBA）可以以Keq = 42M-1的结合常数轻松结合葡萄糖，该结合常数比文献报道的苯基硼酸值大一个数量级。通过荧光发射光谱法显示了1,2-二醇与聚TBA的化学结合。与乙二醇和频哪醇结合的TBA的晶体结构证实了1,2-二醇的结合。 IDE平台通过噻吩3-硼酸（TBA）聚合物通过电化学沉积功能化，用于直接检测1,2-二醇。在暴露于1,2-二醇官能团（例如葡萄糖）时进行电导率测量。 1--10 mM的缓冲（pH 7.4）葡萄糖溶液在聚TBA上的电流增加，这归因于葡萄糖的结合。我们已经通过化学，光学和电化学方法证明了1,2-二醇（例如葡萄糖）直接与聚TBA结合，是传统间接葡萄糖检测方法的替代方法。

著录项

  • 作者

    Caballero, Jonathan Bano.;

  • 作者单位

    University of California, San Diego.$bChemistry.;

  • 授予单位 University of California, San Diego.$bChemistry.;
  • 学科 Chemistry Inorganic.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 123 p.
  • 总页数 123
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 无机化学;
  • 关键词

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