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Design and implementation of a calibrated hyperspectral small-animal imager: Practical and theoretical aspects of system optimization.

机译：校准的高光谱小动物成像仪的设计和实现：系统优化的实践和理论方面。

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摘要

Pre-clinical imaging has been an important development within the bioscience and pharmacology fields. A rapidly growing area within these fields is small animal fluorescence imaging, in which molecularly targeted fluorescent probes are used to non-invasively image internal events on a gross anatomical scale. Small-animal fluorescence imaging has transitioned from a research technique to pre-clinical technology very quickly, due to its molecular specificity, low cost, and relative ease of use. In addition, its potential uses in gene therapy and as a translational technology are becoming evident.;This thesis outlines the development of an alternative modality for small animal/tissue imaging, using hyperspectral techniques to enable the collection of fluorescence images at different excitation and emission wavelengths. In specific, acousto-optical tunable filters (AOTFs) were used to construct emission-wavelength-scanning and excitation-wavelength-scanning small animal fluorescence imagers. Statistical, classification, and unmixing algorithms have been employed to extract specific fluorescent-dye information from hyperspectral image sets. In this work, we have designed and implemented hyperspectral imaging and analysis techniques to remove background autofluorescence from the desired fluorescence signal, resulting in highly specific and localized fluorescence. Therefore, in practice, it is possible to more accurately pin-point the location and size of diagnostic anatomical markers (e.g. tumors) labeled with fluorescent probes. Furthermore, multiple probes can be individually distinguished. In addition to imaging hardware and acquisition and analysis software, we have designed an optical tissue phantom for quality control and inter-system comparison. The phantom has been modeled using Monte Carlo techniques. The culmination of this work results in an understanding of the advantages and complexities in applying hyperspectral techniques to small animal fluorescence imaging. In conclusion, we have identified potential further applications of hyperspectral methods in pre-clinical and clinical imaging.
机译：临床前成像一直是生物科学和药理学领域的重要发展。在这些领域中，快速发展的领域是小型动物荧光成像，其中分子靶向荧光探针用于以大体解剖规模对内部事件进行无创成像。由于其分子特异性，低成本和相对易用性，小动物荧光成像已非常迅速地从研究技术过渡到临床前技术。此外，它在基因治疗和转化技术中的潜在用途正在变得明显。;本文概述了小动物/组织成像的另一种模式的发展，它使用高光谱技术来收集不同激发和发射下的荧光图像波长。具体而言，使用声光可调滤光片（AOTF）来构建发射波长扫描和激发波长扫描小动物荧光成像仪。统计，分类和解混算法已用于从高光谱图像集中提取特定的荧光染料信息。在这项工作中，我们设计并实现了高光谱成像和分析技术，以从所需的荧光信号中去除背景自发荧光，从而产生高度特异性和局限性的荧光。因此，在实践中，有可能更准确地指出用荧光探针标记的诊断解剖学标记（例如肿瘤）的位置和大小。此外，可以分别区分多个探针。除了成像硬件以及采集和分析软件之外，我们还设计了一种光学组织体模，用于质量控制和系统间比较。该模型已使用蒙特卡洛技术进行了建模。这项工作的最终结果是对将高光谱技术应用于小动物荧光成像的优势和复杂性的理解。总之，我们已经确定了高光谱方法在临床前和临床成像中的潜在进一步应用。

著录项

  • 作者

    Leavesley, Silas Josiah.;

  • 作者单位

    Purdue University.;

  • 授予单位 Purdue University.;
  • 学科 Engineering Biomedical.;Physics Optics.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 213 p.
  • 总页数 213
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;光学;
  • 关键词

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