• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Effect of heat acclimation on heat shock protein 72 and interleukin-10 in humans.
【24h】

Effect of heat acclimation on heat shock protein 72 and interleukin-10 in humans.

机译：热适应对人的热休克蛋白72和白介素10的影响。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this dissertation was to document the in vivo kinetics of heat shock protein (Hsp) 72 and interleukin (IL)-10 during heat acclimation (HA). Peripheral blood mononuclear cell (PMBC) and serum Hsp 72, and plasma IL-10 were measured pre- and post-exercise in 10 male and 2 female subjects (mean+/-SD, age: 24+/-4 years, VO 2max: 54.5+/-9.1 ml/kg/min, body fat: 13.6+/-7.6%) during a 10-day HA protocol. Subjects exercised at 56% of VO2max in a 42°C (28% RH) environment for 2 50-minute exercise bouts, separated by a 15-minute rest period. All sessions were completed in 2 weeks. PMBC were isolated before and after each acclimation session on days 1, 6 and 10. Plasma and serum were collected before and after exercise on the 1st and 10th day of HA. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine PBMC Hsp 72 levels during HA and enzyme-linked immunosorbent assays (ELISA) were used to measure IL-10 and Hsp 72 concentrations.;To confirm that subjects were heat acclimated, duration, peak heart rate (HR), peak mean body temperature (MBT), and peak core body temperature (T c) were compared between days 1 and 10 of HA. Dependent t-tests indicated that peak HR, peak MBT, and peak HR were significantly lower on day 10 versus day 1, while mean duration was significantly higher on day 10 versus day 1. There was tendency for PBMC Hsp 72 to increase from pre- to post-exercise on the 1st day of HA (mean+/-SD, 1.0+/-0 vs. 1.6+/-0.6 density units, respectively, p=0.343). A one-way repeated measures ANOVA was used to show that pre-exercise Hsp 72 levels on day 1 were significantly lower compared to the pre- and post-exercise samples on days 6 and 10 (mean+/-SD, day 6: 2.1+/-1.0, 2.2+/-1.0, day 10: 2.0+/-1.3, 2.2+/-1.0 density units, respectively, p<0.05); samples analyzed on days 6 and 10 were not statistically different. There were no significant differences in serum Hsp 72 and plasma IL-10. The sustained elevation of Hsp 72 from days 6 to 10 may be evidence of a cellular HA process, and reveal the functional importance of Hsp 72 in physiological benefits of HA.
机译：本文的目的是记录热适应（HA）过程中热休克蛋白（Hsp）72和白介素（IL）-10的体内动力学。在运动前后分别对10位男性和2位女性受试者进行了外周血单核细胞（PMBC）和血清Hsp 72以及血浆IL-10的测量（平均+/- SD，年龄：24 +/- 4岁，VO 2max：在10天的HA方案中为54.5 +/- 9.1 ml / kg / min，身体脂肪：13.6 +/- 7.6％。受试者在42°C（28％RH）的环境中以VO2max的56％进行运动，进行2次50分钟的运动，并间隔15分钟。所有课程均在2周内完成。在第1、6和10天的每次适应训练前后，分离PMBC。在HA的第1天和第10天运动之前和之后，收集血浆和血清。用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）测定HA期间的PBMC Hsp 72水平，并用酶联免疫吸附测定（ELISA）测量IL-10和Hsp 72浓度。比较HA的第1天和第10天的持续时间，峰值心率（HR），峰值平均体温（MBT）和峰值核心体温（T c）。相关的t检验表明，与第1天相比，第10天的HR峰值，MBT峰值和HR峰值显着降低，而与第1天相比，第10天的平均持续时间显着更高。PBMCHsp 72较前在HA的第一天进行运动后（平均+/- SD，1.0 +/- 0与1.6 +/- 0.6密度单位，p = 0.343）。使用单向重复测量方差分析（ANOVA）显示，与第6天和第10天的运动前和运动后样本相比，第1天的运动前Hsp 72水平显着降低（平均+/- SD，第6天：2.1+ /-1.0、2.2+/-1.0，第10天：分别为2.0 +/- 1.3、2.2 +/- 1.0密度单位，p <0.05）；在第6天和第10天分析的样品没有统计学差异。血清Hsp 72和血浆IL-10没有显着差异。 Hsp 72从第6天到第10天的持续升高可能是细胞HA过程的证据，并揭示了Hsp 72在HA的生理益处中的功能重要性。

著录项

  • 作者

    Yamada, Paulette Marisa M.;

  • 作者单位

    The University of New Mexico.;

  • 授予单位 The University of New Mexico.;
  • 学科 Biology Physiology.;Biology Cell.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 146 p.
  • 总页数 146
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号