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Molecular mechanisms of pyrethroid resistance in the mosquito, Culex quinquefasciatus (Say).

机译：蚊虫中拟除虫菊酯抗药性的分子机制（Say）。

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摘要

Mosquitoes have been the ideal transmitters of a wide variety of disease agents due to their repeated blood feedings over their life span. Culex quinquefasciatus (Say) is an important disease vector in the Southeastern U.S.A. Mechanisms of resistance in mosquitoes have attracted much attention since they help in the elucidation of pathways of resistance development and in the design of novel strategies to prevent or minimize the spread and evolution of resistance.;A mosquito strain of Cx. quinquefasciatus, HAmCq G0, from Huntsville, Alabama, has been established and further selected with permethrin, a pyrethroid insecticide, in the laboratory. The level of resistance to permethrin in the HAmCqG0 strain is 100-fold compared with an insecticide-susceptible mosquito strain S-Lab. Following permethrin selection for 8 generations, the level of resistance to permethrin in HAmCqG8 strain is 3100-fold compared with the S-Lab strain. High levels of resistance to pyrethroid insecticides in HAmCq parental and selected mosquito strains are conferred by multiple mechanisms, including P450 monooxygenase-, hydrolase- and/or GST-mediated detoxification. Incomplete suppression of pyrethroid resistance by synergists, PBO, DEF and/or DEM, suggests that one or more additional mechanisms are involved in overall resistance of HAmCq. The L-to-F kdr mutation of the sodium channel, the target site of pyrethroid insecticides, plays a very important role in pyrethroid resistance. My study has, for the first time, revealed that the transcriptional regulation of the L-to-F kdr mutation of the sodium channel gene through RNA allelic variation and RNA editing is an important mechanism involved in the evolution of kdr-mediated pyrethroid resistance in mosquitoes. Both Southern Blot and DNA sequencing analyses indicate multiple sodium channel genes presented in mosquitoes. The first full-length mosquito sodium channel gene has been cloned and sequenced from mosquitoes. Sequence comparison of the sodium channel gene between resistant and susceptible mosquitoes has revealed several non-silent and silent mutations that correspond with the levels of pyrethroid resistance in mosquitoes. This result suggests the importance of these mutations in altering the sensitivity of the sodium channels to insecticides and in insecticide resistance. Taken together, my research indicates a complexity of the sodium channel gene expression and its role in pyrethroid resistance of insects and provides a new framework to study the sodium channel gene expression regulation in insecticide resistance of mosquitoes.
机译：由于蚊子在整个生命周期中不断地补血，因此它们已成为各种疾病的理想传播者。库蚊（Say）是美国东南部的重要疾病传播媒介，蚊子的抗药性机制已被广泛关注，因为它们有助于阐明抗药性的发展途径，并有助于设计新的策略来预防或最小化其传播和进化。抵抗力。已经建立了来自阿拉巴马州亨茨维尔的quinquefasciatus HAmCq G0，并在实验室中将其与拟除虫菊酯类杀虫剂苄氯菊酯进一步筛选。与对杀虫剂敏感的蚊子菌株S-Lab相比，HAmCqG0菌株对氯菊酯的抗性水平是其100倍。选择苄氯菊酯8代后，与S-Lab菌株相比，HAmCqG8菌株对苄氯菊酯的抗性水平是3100倍。 HAmCq亲本和所选蚊子菌株中对拟除虫菊酯类杀虫剂的高水平抗性是通过多种机制赋予的，包括P450单加氧酶，水解酶和/或GST介导的排毒。增效剂PBO，DEF和/或DEM对拟除虫菊酯抗药性的不完全抑制表明HAmCq的总体抗药性涉及一种或多种其他机制。拟除虫菊酯类杀虫剂的目标位点钠通道的Lto-F kdr突变在拟除虫菊酯抗性中起着非常重要的作用。我的研究首次揭示了钠通道基因L-to-f kdr突变通过RNA等位基因变异和RNA编辑的转录调控是参与kdr介导的拟除虫菊酯抗性进化的重要机制。蚊子。 Southern Blot和DNA测序分析均表明存在于蚊子中的多个钠通道基因。第一个全长的蚊子钠通道基因已经从蚊子中克隆并测序。耐性和易感性蚊子之间钠通道基因的序列比较显示了一些非沉默和沉默突变，这些突变与蚊子中拟除虫菊酯抗性的水平相对应。该结果表明这些突变在改变钠通道对杀虫剂的敏感性和杀虫剂抗性中的重要性。综上所述，我的研究表明钠通道基因表达的复杂性及其在昆虫拟除虫菊酯抗药性中的作用，并为研究钠通道基因表达在蚊子对杀虫剂抗性中的调控提供了新的框架。

著录项

  • 作者

    Xu, Qiang.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Biology Entomology.;Biology Molecular.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 201 p.
  • 总页数 201
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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