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Seasonal and population dynamics and incidence of Xylella fastidiosa in potential insect vectors of almond leaf scorch disease.

机译:杏仁叶焦病潜在昆虫载体中的小生小球藻的季节和种群动态及发生率。

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摘要

In recent years, almond leaf scorch disease (ALSD), caused by Xylella fastidiosa (Xf), has reemerged as a serious threat to almond production areas throughout California's San Joaquin Valley. The primary vector(s) of ALSD, however, has not been well documented. The seasonal population dynamics of green sharpshooter (GSS) (Draculacephala minerva Ball), threecornered alfalfa hopper (Spissistilus festinus Say) and watercress leafhopper (Acinopterus angulatus ) were monitored in permanent, irrigated pastures adjacent to almonds with a high incidence of ALSD. Green sharpshooters were observed to be the predominant known vector species collected in these habitats, with population peaks occurring during summer months (May-September). Threecornered alfalfa hopper and watercress leafhopper were present in comparatively low populations. DNA was extracted from collected insects and single nucleotide polymorphism based polymerase chain reaction was performed to determine the presence and strains of Xf. Infection rates recorded for all three species were relatively high (65-90%) with no seasonal changes.
机译:近年来,由Xylella fastidiosa(Xf)引起的杏仁焦烧病(ALSD)再次出现,严重威胁了加利福尼亚州圣华金河谷的整个杏仁生产区。但是,ALSD的主要载体尚未得到充分记录。在与ALSD发生率高的杏仁相邻的永久性灌溉牧场中,监测了绿色神枪手(GSS)(Draculacephala minerva Ball),三角苜蓿漏斗(Spissistilus festinus Say)和豆瓣菜蝉(Acinopterus angulatus)的季节性种群动态。观察到绿色神枪手是在这些栖息地中收集的主要已知载体物种,在夏季(5月至9月)出现种群高峰。人口相对较少的地区有三corn苜蓿和豆瓣叶蝉。从收集的昆虫中提取DNA并进行基于单核苷酸多态性的聚合酶链反应,以确定Xf的存在和菌株。这三个物种的感染率均相对较高(65-90%),没有季节性变化。

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