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Effect of porcine circovirus type 2 on porcine cell populations.

机译：2型猪圆环病毒对猪细胞群的影响。

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摘要

Porcine circovirus type 2 (PCV2) is an important pathogen of swine and is associated with postweaning multisystemic wasting syndrome which is characterized by follicular and interfollicular lymphoid depletion and a concurrent macrophage infiltration of lymphoid tissues. The mechanism of the lymphocyte depletion in PCV2 infected pigs and the specific immune cell populations in which PCV2 replicates remains unknown. The objectives of these studies were to evaluate PCV2 replication in specific immune cell populations in vitro and in vivo, and investigate the effects of PCV2 infection and replication on activated immune cells in vitro. We demonstrated that mitogen stimulation/activation increases virus replication in peripheral blood mononuclear cells (PBMCs) in vitro and the virus replication level was higher with pokeweed mitogen (PWM) stimulation compared to concanavalin A (ConA) stimulation. PCV2 replicates in T and B lymphocytes, which appear to be the primary cells that support virus replication while monocytes appear to be a site for PCV2 persistence in the infected host. We found that PCV2 replicates in most tissues except thymus and lymphoid tissues near the site of infection play an important role in PCV2 replication and persistence. Our investigation of the effect of PCV2 infection and replication on activated porcine PBMCs in vitro demonstrated that PCV2 infection does not impair the ability of PBMCs to proliferate in response to ConA/PWM stimulation. Increased apoptosis was associated with PCV2 infection in the presence of PWM, but not ConA, indicating that PCV2 induced apoptosis requires a specific stimulation/trigger. In the presence of PCV2 alone, apoptosis was decreased suggesting that PCV2 may persist in immune cells by inhibiting apoptosis. The relationship between virus replication and cellular proliferation was investigated and no significant differences were observed between the levels of PCV2 replication product, spliced capsid mRNA, in PBMCs stimulated at various times prior to PCV2 infection or between proliferating and non-proliferating PBMCs with ConA/PWM stimulation. These findings suggest that PCV2 replication requires a specific stimulation/trigger and is not dependent just on cell division/proliferation. These studies provide important information on understanding the pathogenesis of PCV2-induced diseases.
机译：猪圆环病毒2型（PCV2）是猪的重要病原体，与断奶后多系统衰竭综合征相关，该综合征的特征是滤泡和滤泡间淋巴样耗竭，并发巨噬细胞浸润淋巴样组织。感染PCV2的猪中淋巴细胞耗竭的机制以及PCV2复制的特定免疫细胞群仍然未知。这些研究的目的是评估体外和体内特定免疫细胞群中PCV2的复制，并研究PCV2感染和复制对体外活化的免疫细胞的影响。我们证明有丝分裂原刺激/激活增加体外在外周血单个核细胞（PBMC）中的病毒复制，并且比伴刀豆球蛋白A（ConA）刺激，商陆有丝分裂原（PWM）刺激的病毒复制水平更高。 PCV2在T和B淋巴细胞中复制，T和B淋巴细胞似乎是支持病毒复制的主要细胞，而单核细胞似乎是PCV2在感染宿主中持续存在的位点。我们发现PCV2在除感染部位附近的胸腺和淋巴组织以外的大多数组织中复制，在PCV2复制和持久性中起重要作用。我们对PCV2感染和复制对体外活化猪PBMC的影响的研究表明，PCV2感染不会损害​​PBMC响应ConA / PWM刺激而增殖的能力。在存在PWM的情况下，凋亡增加与PCV2感染相关，但与ConA无关，这表明PCV2诱导的凋亡需要特定的刺激/触发。在单独存在PCV2的情况下，凋亡减少，提示PCV2可能通过抑制凋亡而在免疫细胞中持续存在。研究了病毒复制与细胞增殖之间的关系，在PCV2感染之前的不同时间刺激的PBMC中，ConA / PWM的增殖和非增殖PBMC中，PCV2复制产物，剪接衣壳mRNA的水平之间无显着差异。刺激。这些发现表明，PCV2复制需要特定的刺激/触发，而不仅依赖于细胞分裂/增殖。这些研究为了解PCV2诱导疾病的发病机理提供了重要信息。

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  • 作者

    Yu, Shan.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Biology Microbiology.; Biology Animal Physiology.; Biology Veterinary Science.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 148 p.
  • 总页数 148
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;生理学;动物学;
  • 关键词

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