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Development of a MALDI-ion mobility-surface-induced dissociation-time-of-flight mass spectrometer with novel collision source configurations for high throughput peptide sequencing.

机译：具有高通量肽测序的新型碰撞源配置的MALDI离子迁移率表面诱导的飞行时间解离飞行时间质谱仪的开发。

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A Matrix-assisted Laser Desorption/Ionization (MALDI) - Ion Mobility (IM) - Surface-induced Dissociation (SID) - Time-of-Flight (TOF) instrument with three different collision source configurations was developed in order to improve the SID performance in high throughput peptide sequencing. The first version of the instrument was equipped with an angle resolved SID source in order to maximize the collection efficiency of the SID scattering ions. An orthogonal TOF was also implemented as the second MS stage in this instrument to increase mass resolution. The second version of the instrument was developed towards simplifying the coupled configuration of the IM, SID and TOF components by using a combined SID/TOF source with a confinement ring electrode as the collision target. The fragmentation efficiency of SID in this configuration was increased up to 50% due to the surface normal impact angle used as compared with the results from a previous experiment using 45 degree impact angle. The third version of the instrument was equipped with a dual-source/dual-detector TOF to facilitate high throughput tandem analysis of peptides through simultaneous separation, fragmentation and mass analysis, while retaining precursor ion identity in the same experimental sequence. A series of small organic molecules, model peptides and tryptic peptides from a protein digest were analyzed to demonstrate the utility of these new designs for enhanced SID performance and peptide sequencing capability. Finally, a new mobility drift cell using a periodic focusing mechanism has been designed and fabricated to replace the previous uniform field drift cell. Improvement in ion transmission has been observed in the periodic focusing drift cell instrument without sacrificing the mobility resolution.

机译：为了提高SID性能，开发了具有三种不同碰撞源配置的基质辅助激光解吸/电离（MALDI）-离子迁移率（IM）-表面诱导解离（SID）-飞行时间（TOF）仪器在高通量肽测序中。该仪器的第一个版本配备了角度分辨SID源，以最大化SID散射离子的收集效率。正交TOF也被用作该仪器的第二个MS阶段，以提高质量分辨率。该仪器的第二种版本是通过使用结合了约束环电极的SID / TOF源作为碰撞目标来简化IM，SID和TOF组件的耦合配置而开发的。与先前使用45度冲击角的实验结果相比，由于所使用的表面法向冲击角，在这种配置下SID的碎裂效率提高了50％。该仪器的第三版配备了双源/双检测器TOF，可通过同时分离，片段化和质量分析促进肽的高通量串联分析，同时在相同的实验序列中保留前体离子的身份。分析了一系列来自蛋白质消化物的小分子有机分子，模型肽和胰蛋白酶肽，以证明这些新设计对增强SID性能和肽测序能力的实用性。最后，已经设计并制造了一种使用周期性聚焦机制的新型迁移率漂移单元，以取代以前的均匀场漂移单元。在周期性聚焦漂移池仪器中已经观察到离子传输的改善，而没有牺牲迁移率分辨率。

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Sun, Wenjian.;
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Texas A&M University.;

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授予单位 Texas A&M University.;
学科 Chemistry Analytical.
学位 Ph.D.
年度 2007
页码 129 p.
总页数 129
原文格式 PDF
正文语种 eng
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专利

1. A dual time-of-flight apparatus for an ion mobility-surface-induced dissociation-mass spectrometer for high-throughput peptide sequencing [J] . Sun WJ, May JC, Gillig KJ, International journal of mass spectrometry . 2009,第1a3期

机译：用于高通量肽测序的离子迁移率表面诱导的解离质谱仪的双飞行时间设备
2. Specific screening method for dextran and hydroxyethyl starch in human urine by size exclusion chromatography-in-source collision-induced dissociation-time-of-flight mass spectrometry [J] . Kolmonen M., Leinonen A., Kuuranne T., Analytical and bioanalytical chemistry . 2011,第2期

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3. Surface-Induced Dissociation on a MALDI-Ion Mobility-Orthogonal Time-of-Flight Mass Spectrometer: Sequencing Peptides from an "In-Solution" Protein Digest [J] . Earle Stone, Kent J. Gillig, Brandon Ruotolo, Analytical chemistry . 2001,第10期

机译：MALDI离子迁移率-正交飞行时间质谱仪上的表面诱导解离：从“溶液中”蛋白质文摘中测序肽
4. A Next Generation MALDI-Ion Mobility-Surface-induced Dissociation-Time-of-Flight Mass Spectrometer with Novel Collision Source Configurations [C] . Wenjian Sun, Kent Gillig, Liuxi Chen, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：下一代Maldi离子流动性 - 表面诱导的脱离飞行时间质谱仪，具有新颖的碰撞源配置
5. Development of a MALDI-ion mobility-surface-induced dissociation-time-of-flight-mass spectrometer for the analysis of peptides and protein digests [D] . Stone, Earle Gregory 2003

机译：MALDI离子迁移率表面诱导的飞行时间解离质谱仪的开发，用于分析肽和蛋白质消化物
6. Optimisation of Milk Protein Top-Down Sequencing Using In-Source Collision-Induced Dissociation in the Maxis Quadrupole Time-of-Flight Mass Spectrometer [O] . Delphine Vincent, Dominik Mertens, Simone Rochfort 2018

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7. Development of a MALDI-Ion Mobility-Surface-Induced Dissociation-Time-of-flight-mass spectrometer for the analysis of peptides and proteins [O] . Stone Earle Gregory 2004

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8. Target Gas Effects on Collision-Induced Dissociation of Peptides in a Tandem Four-Sector Mass Spectrometer [R] . Wilson, W. D. 1991

机译：目标气体对串联四扇区质谱中碰撞诱导的肽解离的影响

Development of a MALDI-ion mobility-surface-induced dissociation-time-of-flight mass spectrometer with novel collision source configurations for high throughput peptide sequencing.

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