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Pulsed electrochemical detection of sulfur-containing antibiotics.

机译：脉冲电化学检测含硫抗生素。

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摘要

Sulfur-containing antibiotics are routinely used in both human and veterinary medicine for the treatment of bacterial infections. These compounds are also used in veterinary medicine for growth promotion, as well as for the prevention and treatment of bacterial bee diseases. If proper withdrawal times are not observed, food derived from these animals may be contaminated with residual amounts of antibiotics. The improper and illegal use of drugs will also often result in antibiotic residues in food. The presence of such residues in food is of toxicological and regulatory concern as these may cause allergic hypersensitivity reactions, and may also pose antibiotic resistance in humans.;This research takes advantage of electrochemical detection (ECD), known for its inherent sensitivity and selectivity for electroactive compounds, for the determination of sulfur-containing antibiotics. Pulsed electrochemical detection (PED) with integrated pulsed amperometric detection (IPAD) waveform will be applied here; PED is sensitive, selective, and direct. Cyclic voltammetry (CV) was utilized to optimize the IPAD waveform parameters and to study the electrochemical properties of sulfur-containing antibiotics, as well as primary aromatic amines (PAAs).;The developed waveform was applied for the quantitative determination of sulfonamides (SAs) in pharmaceutical formulations. Results obtained from the PED method were compared to those obtained using UV detection for the quantitative analysis of sulfa drugs in pharmaceutical formulations. HPLC-PED was found to be a valid method for the determination of sulfa drugs in pharmaceutical formulations. The developed waveform was also applied for the determination of sulfathiazole (STZ) and lincomycin in honey. HPLC-PED was found to be a valid method for sensitive and selective determination of the antibiotics in honey. Sample pretreatment was achieved by solid-phase extraction (SPE) using OASIS HLB cartridges. The method detection limits were determined to be 30 mug˙kg-1 and 160 mug˙kg-1 for STZ and lincomycin, respectively. The high sensitivity and selectivity of PED for sulfa drugs and lincomycin reduces sample preparation and produces simpler chromatograms in complex matrices when compared to UV detection. IPAD provides an alternative means of detection for the determination of sulfur-containing antibiotics, many of which have poor chromophoric properties.
机译：含硫抗生素常规用于人类和兽医学，以治疗细菌感染。这些化合物也用于兽药中促进生长，以及预防和治疗细菌性蜜蜂疾病。如果未观察到适当的停药时间，则源自这些动物的食物可能被残留量的抗生素污染。药物的不当和非法使用通常还会导致食物中残留抗生素。食品中此类残留物的存在会引起毒理学和法规方面的关注，因为它们可能引起过敏性超敏反应，并可能对人类造成抗生素耐药性。本研究利用电化学检测（ECD）的优势，其固有的敏感性和选择性对电活性化合物，用于测定含硫抗生素。具有集成脉冲安培检测（IPAD）波形的脉冲电化学检测（PED）将在此处应用； PED是敏感，选择性和直接的。循环伏安法（CV）用于优化IPAD波形参数并研究含硫抗生素以及伯芳族胺（PAA）的电化学性能;所开发的波形用于定量测定磺酰胺（SAs）在药物制剂中。将PED方法获得的结果与使用UV检测获得的结果进行比较，以定量分析药物制剂中的磺胺类药物。发现HPLC-PED是测定药物制剂中磺胺药物的有效方法。所开发的波形还用于测定蜂蜜中的磺胺噻唑（STZ）和林可霉素。发现HPLC-PED是灵敏和选择性测定蜂蜜中抗生素的有效方法。使用OASIS HLB柱通过固相萃取（SPE）实现样品预处理。对于STZ和林可霉素，该方法的检测极限被确定为分别为30马克·kg-1和160马克·kg-1。与紫外检测相比，PED对磺胺类药物和林可霉素的高灵敏度和选择性减少了样品制备，并在复杂基质中产生了更简单的色谱图。 IPAD为测定含硫抗生素提供了另一种检测手段，其中许多具有发色特性差。

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  • 作者

    Vavilala, Suma.;

  • 作者单位

    University of Maryland, Baltimore County.;

  • 授予单位 University of Maryland, Baltimore County.;
  • 学科 Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 149 p.
  • 总页数 149
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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