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Function of abscisic acid in maintenance of maize primary root growth under water deficit.

机译:缺水在缺水条件下维持玉米原根生长的功能。

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摘要

Previous studies suggested that ABA accumulation is required to maintain maize primary root elongation under water deficit and that one of the functions of ABA is to restrict ethylene evolution. These studies were conducted by using carotenoid deficient seedlings to induce ABA deficiency. Using the recently available ABA-deficient mutant vp14, it was confirmed that endogenous ABA accumulation maintains root growth under water stress by restricting ethylene synthesis. Gene expression studies confirmed that the Vp14 is an important regulator of the ABA synthesis required for root growth maintenance under water deficit. Staining for intracellular ROS in vp14 roots indicated that ABA deficiency under water deficit caused excess ROS levels. Moreover, the increase in ROS levels preceded and caused loss of plasma membrane integrity in vp14 roots under water deficits. The results provide conclusive evidence that the maintenance of elongation in the maize primary root requires the accumulation of ABA both to restrict ethylene synthesis and to prevent excess levels of intracellular ROS. A supplemental objective was to develop a method for imaging apoplastic ROS. Increased apoplastic ROS levels occurred specifically in the region of growth maintenance in roots under water deficits. Further work is needed to determine the mechanism by which ABA regulates ROS balance in water-stressed roots.
机译:先前的研究表明,在水分不足的情况下,需要ABA积累来维持玉米的初生根伸长,而ABA的功能之一是限制乙烯的进化。这些研究是通过使用类胡萝卜素不足的幼苗诱导ABA缺乏引起的。使用最近可用的ABA缺失突变体vp14,已证实内源ABA积累通过限制乙烯的合成在水分胁迫下维持根的生长。基因表达研究证实,Vp14是水分缺乏下维持根生长所需的ABA合成的重要调节剂。 vp14根中细胞内ROS的染色表明,水分亏缺下的ABA缺乏会导致ROS过量。此外,在缺水情况下,vp14根中的ROS水平先于并导致质膜完整性丧失。结果提供了确凿的证据,要维持玉米原根的伸长率,就需要积累ABA,以限制乙烯的合成并防止细胞内ROS的过量水平。一个补充目的是开发一种用于成像质外性ROS的成像方法。缺水状态下根部生长维持性ROS水平升高特别发生在根部维持生长的区域。需要进一步的工作来确定ABA调节水分胁迫根中ROS平衡的机制。

著录项

  • 作者

    Cho, In-jeong.;

  • 作者单位

    University of Missouri - Columbia.;

  • 授予单位 University of Missouri - Columbia.;
  • 学科 Agriculture Agronomy.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 152 p.
  • 总页数 152
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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