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首页> 外文学位 >Characterization of Anthropogenic Influence and Levels of Antibiotic Resistance Gene, erm(F), in Soil at 26 Public Parks in Four Cities and Two Pristine Sites in California.
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Characterization of Anthropogenic Influence and Levels of Antibiotic Resistance Gene, erm(F), in Soil at 26 Public Parks in Four Cities and Two Pristine Sites in California.

机译：加利福尼亚州四个城市和两个原始地点的26个公园的土壤中人为影响和抗生素抗性基因erm（F）的水平。

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摘要

The goal of this study was to characterize the soil constitution and the presence of antibiotic resistance gene (ARG) erm(F) in soils across California to investigate the relationship between the levels of anthropogenic influence and ARG erm(F) levels locally and regionally. Soil samples were collected from 26 parks in four cities, San Diego, Los Angeles, Bakersfield, Fresno, where six publicly accessible parks sites were selected in each, and two pristine sites, Yosemite National Park and Mount Baldy. DNA was extracted, normalized, and tested for the presence and quantity of genes 16S rRNA and erm(F) using quantitative polymerase chain reaction (qPCR). Erm(F) quantities were determined via two analysis approaches---on a per gram of soil basis, and relative to 16S gene abundance. Locally among 26 parks, the five quantifiably detected erm(F) sites all occurred in high-level anthropogenic influenced sites. Regionally among four cities, urban and agricultural cities shared the similar soil constitution respectively and urban cities had higher erm(F) gene levels than agricultural ones. Population and population density in each city is positively correlated with erm(F) gene levels whichever analysis approach was applied.
机译：这项研究的目的是表征整个加利福尼亚州土壤的土壤结构和抗生素抗性基因（ARG）erm（F）的存在，以调查人为影响水平与ARG erm（F）水平在局部和区域之间的关系。从圣地亚哥，洛杉矶，贝克斯菲尔德，弗雷斯诺这四个城市的26个公园中采集了土壤样本，每个公园均选择了六个公园，另外两个原始公园分别为优胜美地国家公园和山地秃头。使用定量聚合酶链反应（qPCR）提取DNA，进行标准化，并测试基因16S rRNA和erm（F）的存在和数量。 Erm（F）的数量通过两种分析方法确定-以每克土壤为基础，并相对于16S基因丰度。在26个公园中，五个可定量检测到的erm（F）站点均发生在高水平的人为影响站点中。在四个城市的区域中，城市和农业城市分别具有相似的土壤组成，并且城市中的erm（F）基因水平高于农业城市。无论采用哪种分析方法，每个城市的人口和人口密度都与erm（F）基因水平呈正相关。

著录项

  • 作者

    Sun, Fangfang.;

  • 作者单位

    University of California, Los Angeles.;

  • 授予单位 University of California, Los Angeles.;
  • 学科 Environmental engineering.;Environmental studies.
  • 学位 M.S.
  • 年度 2016
  • 页码 53 p.
  • 总页数 53
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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