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Directly measuring the adhesive and elastic properties of bacteria using a surface force apparatus.

机译：使用表面测力仪直接测量细菌的粘附力和弹性。

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摘要

Bacterial adhesion is the first step of biofilm formation that plays various roles in the environment and the human body. Examples of undesirable roles of biofilm formation include metal rust, sewage sludge and bacteria-related diseases. Desirable roles are biofiltration and bioremediation.;For a decade, Atomic Force Microscopy (AFM) has been the primary tool used to study the adhesion and elastic properties of individual bacteria. In this work we show it is possible to use a Surface Forces Apparatus (SFA) to measure elastic and adhesive properties of small collections of surface bound bacteria. The measurements are conducted with incomplete, patterned bacterial films and we have developed a protocol to image the contact area with AFM after the experiment. Using the SFA, we measured the force profile between a P. Aeruginosa PAO1 film and a bare mica surface. We repeated the measurement in the same contact position for up to ten days to determine the effect of desiccation on the film material properties, and then moved to the new contact area to measure the film thickness and elastic properties. A large shrinkage of the bacterial film thickness was measured during the first few days due to the bacterial film desiccation and rearrangement. The proportion of shrinkage depends on factors such as the bacterial film coverage, roughness, temperature and relative humidity. Thickness compressibility was estimated from the force curves. As a force approximation, the stress at the center of the contact (sigma) and the area of the contact were estimated by applying the Hertz model. Since the film is incomplete the calculated area in contact was reduced by a factor estimated from the optical image of the contact zone. Adhesiveness was measured in receding force profiles. Maximum adhesive force was detected in the first day, due to the high capillary force, decreased by the bacterial film desiccation and increased again due to the conditioning film.
机译：细菌粘附是生物膜形成的第一步，在环境和人体中扮演着各种角色。生物膜形成的不良作用的例子包括金属锈，污水污泥和细菌相关疾病。理想的作用是生物过滤和生物修复。十年来，原子力显微镜（AFM）一直是研究单个细菌的粘附和弹性特性的主要工具。在这项工作中，我们表明可以使用表面力仪器（SFA）来测量少量表面结合细菌的弹性和粘合性能。测量是用不完整的，有图案的细菌膜进行的，实验后，我们开发了一种协议来对与AFM的接触区域成像。使用SFA，我们测量了铜绿假单胞菌PAO1膜和云母裸露表面之间的力分布。我们在相同的接触位置重复测量最多十天，以确定干燥对薄膜材料性能的影响，然后移至新的接触区域以测量薄膜厚度和弹性性能。由于细菌膜的干燥和重排，在最初的几天中细菌膜的厚度大幅度收缩。收缩的比例取决于诸如细菌膜覆盖率，粗糙度，温度和相对湿度等因素。由力曲线估计厚度可压缩性。作为力的近似值，通过应用Hertz模型估算了触点中心的应力（sigma）和触点面积。由于薄膜不完整，因此所计算的接触面积减少了从接触区域的光学图像估算得出的系数。在后退力分布中测量粘合性。在第一天就检测到最大的粘附力，这归因于较高的毛细作用力，其由于细菌膜的干燥而降低，而由于调理膜而再次增加。

著录项

  • 作者

    Heo, Cheol Ho.;

  • 作者单位

    The University of Arizona.;

  • 授予单位 The University of Arizona.;
  • 学科 Environmental Sciences.;Engineering Environmental.;Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 156 p.
  • 总页数 156
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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