• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Evaluation of indicator microbe sampling and enumeration methods for Chicago area surface waters.
【24h】

Evaluation of indicator microbe sampling and enumeration methods for Chicago area surface waters.

机译：评价芝加哥地区地表水的指示剂微生物取样和计数方法。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Three main research objectives were investigated in the area of microbiological analyses and sampling of Chicago surface waters with special regards to the data quality objectives of a comprehensive epidemiological study referred to as "CHEERS" (Chicago Health, Environmental Exposure, and Recreation Study). Two indicator bacteria, E. coli and enterococci and two viral indicators male-specific and somatic coliphages were involved in the studies.;The first study adapted and validated EPA method 1602 (Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure) to the surface water matrices of the CHEERS study. Previously, the EPA method was validated for drinking and ground waters only. The modified method performed well for male-specific coliphages, however slightly underperformed for somatic coliphages if compared to the criteria set for drinking/ground waters.;The second study explored the precision and accuracy of indicator bacteria E. coli and enterococci enumeration data for EPA methods 1600 and 1603. Spiked surface water samples were evaluated at five different processed volumes including all countable plates. The accuracy and precision of plate counts did not decline when colony counts exceeded the EPA-specified upper counting limits (60 colonies for enterococci and 80 colonies for E. coli). The highest processed sample volumes gave the most accurate and precise results for both, E. coli and enterococci bacteria.;The third study developed the proper sampling strategy for the characterization of exposure during secondary contact water recreation in the Chicago Area Waterways based on the spatial and temporal variability of indicator microorganisms. Two preliminary studies and three-month of field sampling with 1900 analytical results were included in the evaluations. Cross-sectional variability of microbe densities was not significant in the upper 10 cm surface of the Chicago waterways.
机译：在芝加哥地表水的微生物分析和采样领域中，调查了三个主要研究目标，并特别关注了被称为“ CHEERS”的全面流行病学研究（芝加哥健康，环境暴露和娱乐研究）的数据质量目标。两项指标细菌分别为大肠杆菌和肠球菌，以及两种病毒指标雄性特异性和体细胞性巨噬细胞;第一项研究采用并验证了EPA方法1602（雄性特异性（F +）和单琼脂在水中的体脂分离） CHEERS研究的地表水基质层（SAL）程序）。以前，EPA方法仅在饮用水和地下水中得到验证。修改后的方法对男性特异的噬菌体效果很好，但是与饮用水/地下水标准相比，对于体细胞的噬菌体表现稍差。;第二项研究探讨了指示性细菌大肠杆菌的准确性和准确性以及EPA的肠球菌计数数据方法1600和1603。以五个不同的处理体积（包括所有可计数板）对掺加的地表水样品进行了评估。当菌落计数超过EPA指定的计数上限（肠球菌为60个菌落，大肠杆菌为80个菌落）时，平板计数的准确性和精密度不会下降。处理后的最大样品量为大肠杆菌和肠球菌均提供了最准确和最精确的结果。第三项研究开发了适当的采样策略，以根据空间上的特征确定芝加哥地区水道二次接触水游憩期间暴露的特征指示微生物的时空变化。评估包括两项初步研究和为期三个月的实地采样，分析结果均为1900个。在芝加哥水道的上部10 cm表面，微生物密度的横截面变异性不显着。

著录项

  • 作者

    Javor, Margit.;

  • 作者单位

    University of Illinois at Chicago, Health Sciences Center.;

  • 授予单位 University of Illinois at Chicago, Health Sciences Center.;
  • 学科 Biology Microbiology.;Environmental Health.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 344 p.
  • 总页数 344
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号