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首页> 外文学位 >Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos.
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Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos.

机译：体外产生的牛胚胎培养过程中的能量底物，代谢调节剂和脂质蓄积。

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摘要

The main objective of this experiment was to optimize in vitro culture conditions for bovine embryonic development, using alternative energy sources and metabolic regulators. Replacing glucose with fructose in culture medium consistently increased blastocyst production per oocyte and decreased lipid content in bovine embryos. The use of phenazine ethosulphate (PES) or fetal calf serum (FCS) supplementation did not affect embryonic development; however, PES consistently decreased, and FCS increased lipid content of embryos compared to the control. There was no effect of glucose or fructose on survival of embryos after cryopreservation by slow freezing or vitrification; however, embryos-treated with PES to reduce lipid content resulted in improved cryotolerance, and FCS decreased cryotolerance compared to the control. Transfer of embryos treated with PES during in vitro culture did not affect pregnancy rates, conceptus losses, or fetal or post-natal development in calves born normally. The sex ratio of calves born was skewed toward males. This effect likely was due to a toxic effect of glucose to female embryos cultured in vitro. Therefore, the more expanded day 7 blastocysts were mostly male embryos.;A new, objective and less time consuming technique to quantify lipid accumulation using fluorescence of Nile red dye was validated. The progression of the early to expanded blastocyst resulted in decreased lipid content; also, the inner cell mass accumulated more lipids than the trophoblast compartment. Embryos were treated with various lipolytic agents. Forskolin reduced lipid content of embryos relative to controls, but caffeine and epinephrine did not affect lipid content of embryos at the doses tested. None of the lipolytic agents affected embryonic development except that high doses of caffeine were detrimental. A higher a percentage of oocytes derived from cow than post-pubertal heifer ovaries developed into blastocyst in vitro; however, more good quality oocytes were recovered per heifer ovary.
机译：该实验的主要目的是使用替代能源和代谢调节剂优化牛胚胎发育的体外培养条件。在培养基中用果糖代替葡萄糖可以持续增加每个卵母细胞的胚泡产量，并降低牛胚胎中的脂质含量。使用吩嗪乙醇硫酸盐（PES）或胎牛血清（FCS）补充剂不会影响胚胎发育。然而，与对照组相比，PES持续下降，而FCS增加了胚胎的脂质含量。通过缓慢冷冻或玻璃化冷冻保存后，葡萄糖或果糖对胚胎的存活没有影响。然而，与对照组相比，用PES处理以降低脂质含量的胚胎可提高耐低温性，而FCS可以降低耐低温性。在体外培养过程中用PES处理过的胚胎的转移不会影响正常出生的小牛的妊娠率，概念损失或胎儿或产后发育。小牛出生时的性别比例偏向男性。该作用可能是由于葡萄糖对体外培养的雌性胚胎具有毒性作用。因此，第7天囊胚的膨胀更多为雄性胚。验证了一种新颖，客观，耗时少的利用尼罗红染料荧光定量脂质积累的技术。胚泡从早期到膨胀的发展导致脂质含量降低。同样，内部细胞团比滋养层室积累更多的脂质。胚胎用各种脂解剂处理。佛司可林相对于对照组降低了胚胎的脂质含量，但是咖啡因和肾上腺素在所测试的剂量下不会影响胚胎的脂质含量。除高剂量的咖啡因有害外，没有任何脂解剂影响胚胎发育。体外发育为胚泡的青春期后小母牛的卵巢中卵母细胞的百分比更高；然而，每个小母牛卵巢中回收的卵母细胞质量更高。

著录项

  • 作者

    Barcelo-Fimbres, Moises.;

  • 作者单位

    Colorado State University.;

  • 授予单位 Colorado State University.;
  • 学科 Biology Animal Physiology.;Engineering Environmental.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 266 p.
  • 总页数 266
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;环境污染及其防治;
  • 关键词

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