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首页> 外文学位 >Microbial decolorization of distillery effluent for its application in wasteland reclamation.
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Microbial decolorization of distillery effluent for its application in wasteland reclamation.

机译：酒糟废水的微生物脱色及其在荒地开垦中的应用。

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摘要

Distillery wastewater refers to the effluent generated during alcohol production and pollution caused by it is one of the most critical environmental issue. Existing methods of effluent treatment use more quantity of water and they are either pollutant specific, less efficient and more expensive than simple discharge without any effluent treatment.;Present work focused on isolating strains of microorganisms from effluent contaminated sites capable of decolorizing the distillery effluent. Characterization of suitable isolates was done. The effect of pH, temperature and effluent concentration on growth of the selected fungal isolates was investigated. Besides, efforts were made to enhance the enzymatic activity of selected fungal isolates by choosing different growth substrates. An attempt was made to reduce the high nitrogen content in the distillery wastewater, which was identified as a deterrent in the growth of fungi at higher concentration of the effluent using a novel hydroponic treatment step before its treatment by immobilized fungi. The real industrial wastewater was treated employing the microbial consortia developed in this study to confirm its efficacy in decolorization of these recalcitrant wastewaters. Finally an attempt was made towards mass production of these isolates for further application in consortium mode in an improvised biological contactor.;A total of twenty-one different fungal strains were obtained from soil and effluent samples. Using morphologic and genetic characterization, the selected isolates were identified as Penicillium pinophilum TERIDB1, Alternaria gaisen TERIDB6, Aspergillus flavus TERIDB9, Fusarium verticillioides TERIDB16, Aspergillus niger TERIDB18 and Aspergillus niger TERIDB20. The optimum temperature ranged from 25 °C to 37 °C and the optimum pH ranged from 4.0 to 10.0. High nitrogen reduction in the effluent was obtained using two plant species, Phragmites kharka and Vetivereia zizanoides. When this hydroponically treated effluent at 50% dilution was subjected to immobilized fungal biomass, decolorization up to 86.33% by Pleurotus florida EM1303 was achieved. Immobilization of the fungi on the solid substrate was found to be effective in enhancing the ligninolytic activity of the fungi. Agriculture residues such as wheat straw and corncob powder were used as a support material. Using scanning electron microscopy, the degradation of wheat straw while fungal growth was observed. Enzymes extracted from solid state fermentation were concentrated using a 10 KDa filter and further used for effluent decolorization. Bench-scale effluent treatment employing the six fungal isolates was attempted in an improvised bioreactor and substantial decolorization and COD removal could be observed even on absolutely undiluted effluent.
机译：酒厂废水是指酒精生产过程中产生的废水，由其引起的污染是最关键的环境问题之一。现有的废水处理方法使用大量的水，它们比不进行任何废水处理的简单排放所特有的污染物，效率更低，成本更高。;目前的工作重点是从能够使酒厂废水脱色的废水污染场所中分离出微生物菌株。对合适的分离物进行了表征。研究了pH，温度和流出物浓度对所选真菌分离物生长的影响。此外，通过选择不同的生长底物，努力增强所选真菌分离物的酶活性。尝试减少酿酒厂废水中的高氮含量，这被认为是在通过固定化真菌处理之前，采用新颖的水培处理步骤，以较高浓度的废水抑制了真菌的生长。使用本研究中开发的微生物联盟对实际工业废水进行了处理，以确认其在这些难处理废水脱色中的功效。最后，人们尝试大量生产这些分离物，以便在改良的生物接触器中以联合体的形式进一步应用。；从土壤和废水样品中获得了总共二十一种不同的真菌菌株。使用形态学和遗传学特征，将选定的分离物鉴定为嗜松青霉TERIDB1，盖氏链格孢菌TERIDB6，黄曲霉TERIDB9，小镰刀菌TERIDB16，黑曲霉TERIDB18和黑曲霉TERIDB20。最佳温度为25°C至37°C，最佳pH为4.0至10.0。使用两种植物芦苇（Phragmites kharka）和香根草（Vetivereia zizanoides）可实现废水中氮的高度还原。当将此经水培处理的稀释液（浓度为50％）进行固定化真菌生物量处理时，佛罗里达侧耳菌EM1303的脱色率最高可达86.33％。发现将真菌固定在固体基质上可有效增强真菌的木质素分解活性。农业残留物如小麦秸秆和玉米芯粉被用作支撑材料。使用扫描电子显微镜，观察到麦草降解而真菌生长。使用10 KDa过滤器浓缩从固态发酵中提取的酶，并进一步用于废水脱色。尝试在简易的生物反应器中使用六种真菌分离物进行台式规模的废水处理，即使在绝对未稀释的废水中，也可以观察到明显的脱色和COD去除。

著录项

  • 作者

    Pant, Deepak.;

  • 作者单位

    TERI University.;

  • 授予单位 TERI University.;
  • 学科 Engineering Chemical.;Engineering Environmental.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 295 p.
  • 总页数 295
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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