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Improved resistance to insects in maize (Zea mays L.) and cowpea (Vigna unguiculata L.).

机译:增强了对玉米(Zea mays L.)和cow豆(Vigna unguiculata L.)昆虫的抗性。

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摘要

A novel alteration in secondary metabolism of maize transformed with wheat oxalate oxidase gene (OXO) was determined using HPLC and microscopy. Phenolic concentration was significantly increased, but DIMBOA synthesis was down-regulated. The high levels of soluble phenolic acids, in particular free ferulic acid, most likely contributed to the insect resistance in the OXO maize.{09}To facilitate future cowpea transformation with the OXO, a stable in vitro regeneration system was established in blackeye cowpea via shoot organogenesis. The optimal initiation medium comprised MS salts, B 5 vitamins, 2 mg 1-1 BAP, 3% sucrose and 0.8% agar at pH 5.8. The highest initiation frequency and shoot number were obtained from the shoot apices of 3-5 days old seedlings. For shoot elongation, 0.5-5.0 mg 1-1 GA3 was required. Rooting medium was MS salts supplemented with B5 vitamins, 3% sucrose and 0.8% agar.
机译:使用HPLC和显微镜确定了小麦草酸氧化酶基因(OXO)转化的玉米次生代谢的新变化。酚浓度显着增加,但DIMBOA合成被下调。高含量的可溶性酚酸,尤其是游离阿魏酸,很可能有助于OXO玉米的抗虫性。{09}为了促进将来用OXO转化cow豆,在黑眼cow豆中建立了稳定的体外再生系统射击器官发生。最佳起始培养基包括MS盐,B 5维生素,2 mg 1-1 BAP,3%蔗糖和0.8%pH 5.8的琼脂。从3-5天龄幼苗的芽尖获得最高的起始频率和芽数。对于枝条伸长,需要0.5-5.0mg 1-1 GA 3。生根培养基是补充了B5维生素,3%蔗糖和0.8%琼脂的MS盐。

著录项

  • 作者

    Mao, Jingqin.;

  • 作者单位

    University of Ottawa (Canada).;

  • 授予单位 University of Ottawa (Canada).;
  • 学科 Agriculture Plant Pathology.; Biology Plant Physiology.
  • 学位 M.Sc.
  • 年度 2006
  • 页码 105 p.
  • 总页数 105
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;植物学;
  • 关键词

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