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首页> 外文学位 >The effect of enamel matrix derivative on protein kinase C isoforms expressions in cultured osteoblastic cells.
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The effect of enamel matrix derivative on protein kinase C isoforms expressions in cultured osteoblastic cells.

机译：釉质基质衍生物对培养的成骨细胞中蛋白激酶C同工型表达的影响。

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摘要

Emdogain, an enamel matrix derivative (ENID), has been advocated for use in periodontal regeneration, as an alternative to conventional periodontal therapy, in an effort to try to mimic the events that take place during dental root development. In order to delineate possible intracellular signal transduction pathways involved in this process, we determined the effect of EMD on Protein Kinase C (PKC) isoform expression during the differentiation of MC3T3-E1. Results. EMD down-regulated the expression of PKC alpha and eta compared to controls. Cells in osteogenic media and EMD showed up to two fold increase in PKC alpha expression whereas there was up to two fold decrease in the expression of PKC eta at day 14 as compared to control. Stimulation with EMD up regulates Collagen (Col) gene expression in day 1 that gradually decreased through day 21. There was a twofold increase in Bone sialoprotein (BSP) expression at Day 14 and a maximum level of expression of Osteocalcin (OCN) at day 21. PKC alpha and eta are mainly located in the nucleus of the cells, upon stimulation with ENID, PKC alpha and eta was translocated from the nucleus and re-distribution throughout the cytoplasm. Conclusion. EMD has differential as well as biphasic effects on the differentiating osteoblast. The effect of ENID on osteoblastic cells is culture condition dependent. ENID also has differential effects depending on the differentiation phase of the osteoblast. (Abstract shortened by UMI.)
机译：Emdogain是一种釉质基质衍生物（ENID），已被提倡用于牙周再生，以替代常规的牙周治疗，以试图模仿牙根发育过程中发生的事件。为了描述可能参与此过程的细胞内信号转导途径，我们确定了EMD对MC3T3-E1分化过程中蛋白激酶C（PKC）同工型表达的影响。结果。与对照相比，EMD下调了PKCα和eta的表达。与对照组相比，成骨培养基和EMD中的细胞显示PKCα表达最多增加2倍，而PKC eta表达最多减少2倍。用EMD刺激可在第1天逐渐降低胶原蛋白（Col）基因的表达，直到第21天逐渐减少。第14天，骨唾液蛋白（BSP）的表达增加了两倍，而在第21天，骨钙蛋白（OCN）的表达达到最大水平。 PKCα和eta主要位于细胞核中，经ENID刺激后，PKCα和eta从核中转移出来，并重新分布在整个细胞质中。结论。 EMD对分化的成骨细胞具有差异性和双相性作用。 ENID对成骨细胞的作用取决于培养条件。根据成骨细胞的分化阶段，ENID也具有差异作用。 （摘要由UMI缩短。）

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  • 作者

    Alsilmi, Amal.;

  • 作者单位

    State University of New York at Buffalo.;

  • 授予单位 State University of New York at Buffalo.;
  • 学科 Health Sciences Dentistry.
  • 学位 M.S.
  • 年度 2006
  • 页码 78 p.
  • 总页数 78
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 口腔科学;
  • 关键词

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