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Geometric patterns on silicone surfaces influence bacterial biofilm formation.

机译:硅树脂表面上的几何图案会影响细菌生物膜的形成。

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摘要

Bacteria have the ability to attach to surfaces forming complex communities known as biofilms. Biofilm formation occurs on a wide variety of surfaces including living tissues and indwelling medical devices. Biofilms on implantable devices are a common problem and often the cause of implant rejection and removal. One of the approaches used to prevent biofilm formation on medical implants is the modification of the synthetic polymers used to fabricate medical implants to inhibit bacterial attachment. In this work, we assessed the influence of seven patterned silicone surfaces on Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa biofilm formation. Patterns tested consisted of bars, squares and circles with 6 mum of depth and periodicity between 0.2mum and 20mum. Bacterial biofilm formation on plugs cut from patterned silicone was assessed by direct microscopic observation and quantified as the surface area of pattern covered by biofilm incubation ranging between 20 and 120 hrs. The results obtained for all patterns were compared to those obtained for un-patterned silicone. We found that pattern introduction on silicone surfaces significantly reduced S. aureus. S. epidermidis and P. aeruginosa initial biofilm formation and S. epidermidis later biofilm formation in vitro.
机译:细菌具有附着在表面上的能力,从而形成称为生物膜的复杂群落。生物膜形成发生在各种各样的表面上,包括活组织和留置的医疗设备。植入式设备上的生物膜是一个普遍的问题,通常是植入物被排斥和去除的原因。防止医学植入物上生物膜形成的方法之一是对用于制造医学植入物的合成聚合物进行改性以抑制细菌附着。在这项工作中,我们评估了七个有图案的有机硅表面对金黄色葡萄球菌,表皮葡萄球菌和铜绿假单胞菌生物膜形成的影响。测试的图案由条形,正方形和圆形组成,深度为6毫米,周期为0.2毫米至20毫米。通过直接显微镜观察评估从图案化的有机硅切下的栓塞上的细菌生物膜形成,并将其量化为生物膜温育覆盖的图案表面积在20到120小时之间。将所有图案获得的结果与未图案化硅酮获得的结果进行比较。我们发现在硅树脂表面上引入图案可显着减少金黄色葡萄球菌。表皮葡萄球菌和铜绿假单胞菌最初在体外形成生物膜,而表皮葡萄球菌随后在体外形成生物膜。

著录项

  • 作者

    Molina, Guimel.;

  • 作者单位

    The University of Texas at Arlington.;

  • 授予单位 The University of Texas at Arlington.;
  • 学科 Biology Microbiology.
  • 学位 M.S.
  • 年度 2006
  • 页码 45 p.
  • 总页数 45
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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