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首页> 外文学位 >Formation and characterization of hybrid bilayers and diffusion of cations across liposomal membranes: Studies based on polymerizable lipids .
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Formation and characterization of hybrid bilayers and diffusion of cations across liposomal membranes: Studies based on polymerizable lipids .

机译：杂化双层的形成和表征以及阳离子跨脂质体膜的扩散：基于可聚合脂质的研究。

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摘要

Cellular energy transduction processes are often driven by transmembrane ion gradients, and a number of artificial membrane systems have been developed that allow for chemically or light-induced transport of ions across lipid bilayers. These liposomal architectures, however, are not readily interfaced to a solid-state transducer. A significant step toward this goal is described here by assessing the possibility of coupling a lipid bilayer directly to a transducer to form a stable uniform film using hybrid bilayer membranes (HBMs).; Although the surface attachment of self-assembled monolayer increases the robustness of the lipid assembly, HBMs cannot maintain film uniformity under harsher conditions due to the absence of strong lipid-lipid interactions. Therefore, HBMs were prepared and characterized using a cross-linking polymerizable lipid, bis-SorbPC. Several parameters relating to lipid deposition and film stabilization through polymerization were examined. Film characterization strongly suggests that polymerization of bis-SorbPC stabilizes the HBM such that its structure is largely preserved even after the dehydration process. This work suggests that network formation in the upper monolayer is not enough to prevent oligomer desorption, intermonolayer covalent linking is also a prerequisite in making uniform, defect-free planar supported lipid assemblies.; Some of the challenges associated with the application of lipids involve the creation of supported bilayers that are stable to chemical and physical disruptions, yet retain their ion barrier properties, and allow transmembrane ion transport by lipid-soluble shuttles. Polymerized lipid films provide the stability required for these structures, but permeability properties of cations across poly(lipid) membranes are not known. Therefore, convenient liposome-based proton and Ca2+ permeability assays were developed. These assays were applied to various poly(lipid) compositions.; In addition, three novel sorbyl-substituted head group polymerizable lipids, which have been synthesized based on a strategy that head group polymerization would minimally perturb the characteristic ion impermeability of the membrane, were evaluated for their lipid characteristics and ability to form polymers. None of these compounds forms vesicles by itself. Therefore, attempts were made to form mixed vesicles with other fluid lipids. The miscibility of the mixed monolayers was assessed using Langmuir isotherms.
机译：细胞能量转导过程通常由跨膜离子梯度驱动，并且已经开发了许多人工膜系统，这些系统允许化学或光诱导的离子跨脂质双层传输。但是，这些脂质体结构不容易与固态换能器连接。通过评估使用混合双层膜（HBM）将脂质双层直接耦合到换能器以形成稳定的均匀膜的可能性，此处描述了朝着这个目标迈出的重要一步。尽管自组装单分子层的表面附着增加了脂质组装体的坚固性，但由于缺乏强烈的脂质-脂质相互作用，HBMs无法在更苛刻的条件下保持膜的均匀性。因此，使用可交联的可聚合脂质bis-SorbPC来制备和表征HBM。检查了与脂质沉积和通过聚合作用稳定薄膜有关的几个参数。膜表征强烈表明，bis-SorbPC的聚合作用可稳定HBM，从而即使在脱水过程后仍可很大程度上保留其结构。这项工作表明，在上部单层中形成网络不足以防止低聚物解吸，单层间共价连接也是制造均匀，无缺陷的平面支撑脂质组装体的先决条件。与脂质的应用相关的一些挑战涉及产生对化学和物理破坏稳定的但仍保留其离子屏障性质并允许脂质可溶穿梭进行跨膜离子运输的支撑双层。聚合脂质膜提供了这些结构所需的稳定性，但是阳离子在聚脂膜上的渗透性尚不明确。因此，开发了方便的基于脂质体的质子和Ca2 +渗透性测定。这些测定法适用于各种聚脂组合物。此外，评估了三种新型的山梨糖基取代的头基可聚合脂质，它们是基于头基聚合将最小程度地扰动膜的特征离子不渗透性的策略合成的，其脂质特性和形成聚合物的能力得到了评估。这些化合物均不能单独形成囊泡。因此，试图与其他流体脂质形成混合的囊泡。使用Langmuir等温线评估了混合单层的混溶性。

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  • 作者

    Ratnayaka, Saliya Nalin.;

  • 作者单位

    The University of Arizona.;

  • 授予单位 The University of Arizona.;
  • 学科 Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 325 p.
  • 总页数 325
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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