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An active microscaffold system with fluid delivery and stimulation/recording functionalities for culturing three-dimensional in vitro neuronal networks.

机译：具有流体输送和刺激/记录功能的主动微支架系统，用于培养三维体外神经元网络。

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摘要

The work presented in this dissertation builds upon the conventional concept of passive scaffolds for cell culturing by the addition of active functions (e.g., fluid perfusion, electrodes) integrated directly into the scaffold. Overall, the primary objective of this research was to provide neuroscientists with a better analytical tool for studying 3-D in vitro neuronal networks than was currently available, and was executed through the development of BioMEMS technologies that assisted in the growth, proliferation, and stimulation/recording of 3-D in vitro neuronal networks.; The design, fabrication, and characterization of an active microscaffold system with fluid perfusion and electrical stimulation/recording functionalities for culturing 3-D in vitro neuronal networks is presented. The active microscaffold system consists of an array of microfabricated towers (1-2 mm in height) with integrated fluid perfusion functionality to provide nutrients and aeration to the network of cells growing within the microscaffold, in addition to electrodes for stimulation/recording of the neuronal network. The microtowers provide a structural support system for the neurons to adhere to, enabling branching and network formation with other neurons in the culture. The microtower electrodes, placed at varying heights on the surface of the microtowers, allow for stimulation and recording of the cultured network in a 3-D multielectrode array (MEA) environment.; An active microscaffold system for culturing re-aggregate networks formed from dissociated neurons was designed, fabricated, packaged, and tested, and the biological data gathered from this active microscaffold system demonstrated that the fluid perfusion functionality of the system increased cell viability at 14 and 21 DIV. Additionally, a separate active microscaffold system for culturing thick (≤1 mm) slices of brain tissue was designed, fabricated, and packaged.
机译：本论文提出的工作建立在用于细胞培养的被动支架的传统概念的基础上，方法是增加直接整合到支架中的主动功能（例如，流体灌注，电极）。总体而言，这项研究的主要目的是为神经科学家提供比目前可用的更好的分析工具，以研究3-D体外神经元网络，并通过开发有助于生长，增殖和刺激的BioMEMS技术来执行。 /记录3-D体外神经元网络。介绍了用于培养3-D体外神经元网络的具有流体灌注和电刺激/记录功能的主动微支架系统的设计，制造和表征。有源微支架系统由一系列微结构塔架（高度为1-2 mm）组成，具有集成的流体灌注功能，除了用于刺激/记录神经元的电极外，还为微支架内生长的细胞网络提供营养和通气。网络。微型塔为神经元提供了结构支持系统，使其能够与培养物中的其他神经元分支并形成网络。微型塔电极以不同的高度放置在微型塔的表面上，从而可以在3-D多电极阵列（MEA）环境中刺激和记录培养的网络。设计，制造，包装和测试了一种用于培养由解离的神经元形成的重新聚集网络的主动微支架系统，从该主动微支架系统收集的生物学数据表明，该系统的液体灌注功能提高了14和21岁时的细胞活力。 DIV。另外，设计，制造和包装了用于培养厚（≤1mm）厚的脑组织切片的单独的主动微支架系统。

著录项

  • 作者

    Rowe, Laura Elizabeth.;

  • 作者单位

    Georgia Institute of Technology.;

  • 授予单位 Georgia Institute of Technology.;
  • 学科 Engineering Electronics and Electrical.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 240 p.
  • 总页数 240
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 无线电电子学、电信技术;
  • 关键词

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