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首页> 外文学位 >Microbial Electrochemical Cells for Selective Enrichment and Characterization of Photosynthetic and Haloalkaliphilic Anode-Respiring Bacteria.
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Microbial Electrochemical Cells for Selective Enrichment and Characterization of Photosynthetic and Haloalkaliphilic Anode-Respiring Bacteria.

机译：选择性富集和表征光合和嗜盐阳极呼吸细菌的微生物电化学细胞。

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摘要

Microbial electrochemical cells (MXCs) are promising platforms for bioenergy production from renewable resources. In these systems, specialized anode-respiring bacteria (ARB) deliver electrons from oxidation of organic substrates to the anode of an MXC. While much progress has been made in understanding the microbiology, physiology, and electrochemistry of well-studied model ARB such as Geobacter and Shewanella, tremendous potential exists for MXCs as microbiological platforms for exploring novel ARB. This dissertation introduces approaches for selective enrichment and characterization of phototrophic, halophilic, and alkaliphilic ARB. An enrichment scheme based on manipulation of poised anode potential, light, and nutrient availability led to current generation that responded negatively to light. Analysis of phototrophically enriched communities suggested essential roles for green sulfur bacteria and halophilic ARB in electricity generation. Reconstruction of light-responsive current generation could be successfully achieved using cocultures of anode-respiring Geobacter and phototrophic Chlorobium isolated from the MXC enrichments. Experiments lacking exogenously supplied organic electron donors indicated that Geobacter could produce a measurable current from stored photosynthate in the dark. Community analysis of phototrophic enrichments also identified members of the novel genus Geoalkalibacter as potential ARB. Electrochemical characterization of two haloalkaliphilic, non-phototrophic Geoalkalibacter spp. showed that these bacteria were in fact capable of producing high current densities (4-8 A/m2) and using higher organic substrates under saline or alkaline conditions. The success of these selective enrichment approaches and community analyses in identifying and understanding novel ARB capabilities invites further use of MXCs as robust platforms for fundamental microbiological investigations.
机译：微生物电化学电池（MXC）是利用可再生资源生产生物能源的有前途的平台。在这些系统中，专门的阳极呼吸细菌（ARB）将电子从有机底物的氧化作用传递到MXC的阳极。尽管在深入研究的模型ARB（例如Geobacter和Shewanella）的微生物学，生理学和电化学方面已取得了很大进展，但MXC作为探索新型ARB的微生物学平台仍具有巨大潜力。本文介绍了对光养，嗜盐和嗜碱ARB进行选择性富集和表征的方法。基于操纵阳极势，光和养分利用率的富集方案导致产生了对光产生负面反应的电流。对富营养化群落的分析表明，绿色硫细菌和嗜盐ARB在发电中具有重要作用。通过使用从MXC浓缩物中分离出的可吸入阳极的土杆菌和光养绿藻的共培养，可以成功实现光响应电流的重建。缺乏外源提供的有机电子供体的实验表明，Geobacter可以从黑暗中存储的光合产物中产生可测量的电流。富营养化富集的社区分析还确定了新型地碱杆菌属的成员为潜在的ARB。两种卤代嗜碱，非光养性嗜碱菌属细菌的电化学表征。结果表明，这些细菌实际上能够产生高电流密度（4-8 A / m2），并在盐水或碱性条件下使用更高的有机底物。这些选择性富集方法和社区分析在识别和理解新颖的ARB功能方面的成功经验，进一步邀请了MXC作为基础微生物研究的可靠平台。

著录项

  • 作者

    Badalamenti, Jonathan P.;

  • 作者单位

    Arizona State University.;

  • 授予单位 Arizona State University.;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 146 p.
  • 总页数 146
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:40:44

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