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Roles of WRKY proteins in mediating the crosstalk of hormone signaling pathways: An approach integrating bioinformatics and experimental biology.

机译：WRKY蛋白在介导激素信号传导通路的串扰中的作用：一种整合生物信息学和实验生物学的方法。

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The goal of my research is to understand the molecular mechanism by which hormones control seed germination. Gibberellins (GA) promote, while abscisic acid (ABA) and salicylic acid (SA) inhibit seed germination. Key molecules in these signaling pathways include receptors, secondary messengers, protein kinases and phosphatases, and transcription factors. My study focuses on how WRKY transcription factors modulate the expression of an α-amylase gene (Amy32b), which is up-regulated by GA, but down-regulated by ABA and SA in the aleurone cells of germinating seeds.;Chapter 2 started with the annotation and phylogenetic analyses of the WRKY gene superfamily, followed by functional studies of WRKY proteins in mediating ABA responses. Eighty-one WRKY genes were identified in the rice genome through computational analyses. Phylogenetic analyses based on WRKY domain sequences suggest that extensive duplications and losses of the WRKY domain occurred during evolution of this gene family. Transient expression studies suggest that among four WRKY genes that are ABA-inducible in aleurone cells, OsWRKY72 and OsWRKY77 function as transcription activators while OsWRKY24 and OsWRKY45 are repressors of the ABA-inducible HVA22 gene.;Chapter 3 presents cellular and biochemical data to support a novel model that two transcription repressors OsWRKY51 and OsWRKY71 mediate the crosstalk of GA and ABA signaling. Both genes are ABA-inducible, but GA-repressible in the embryos and aleurone cells of germinating rice seeds. The interaction of OsWRKY51 and OsWRKY71 synergistically represses the GA-induced Amy32b expression, likely by functionally interfering with the GA-inducible transactivator, GAMYB.;The mechanism underlying the crosstalk of SA, ABA and GA is reported in Chapter 4. Similar to ABA, SA blocks seed germination likely through repressing the expression of α-amylase genes such as Amy32b. Over-expression of the SA-inducible and GA repressible HvWRKY38 in aleurone cells blocks GA-induced Amy32b expression. Therefore, HvWRKY38 might mediate the crosstalk of SA, ABA and GA signaling in regulating α-amylase expression and seed germination.;My research demonstrates the high efficiency of the approach integrating bioinformatics and experimental biology in addressing the cell-biological signaling network. The finding derived from this research helps advance towards achieving our long-term goal: to improve the yield and quality of rice and other cereals.
机译：我研究的目的是了解激素控制种子萌发的分子机制。赤霉素（GA）促进，而脱落酸（ABA）和水杨酸（SA）抑制种子发芽。这些信号传导途径中的关键分子包括受体，二级信使，蛋白激酶和磷酸酶以及转录因子。我的研究集中在WRKY转录因子如何调节α-淀粉酶基因（Amy32b）的表达上，该基因被GA上调，而被ABA和SA下调在发芽种子的糊粉细胞中。第二章从WRKY基因超家族的注释和系统发育分析，然后研究WRKY蛋白在介导ABA反应中的功能。通过计算分析，在水稻基因组中鉴定出81个WRKY基因。基于WRKY域序列的系统进化分析表明，在该基因家族的进化过程中，发生了WRKY域的大量重复和丢失。瞬时表达研究表明，在糊粉细胞中可被ABA诱导的四个WRKY基因中，OsWRKY72和OsWRKY77充当转录激活因子，而OsWRKY24和OsWRKY45是ABA诱导的HVA22基因的阻遏物。第3章介绍细胞和生化数据以支持新型模型，两个转录阻遏物OsWRKY51和OsWRKY71介导GA和ABA信号的串扰。这两个基因均可诱导ABA，但在发芽水稻种子的胚芽和糊粉细胞中可抑制GA。 OsWRKY51和OsWRKY71的相互作用可协同抑制GA诱导的Amy32b表达，这可能是功能上干扰了GA诱导的反式激活因子GAMYB。有关SA，ABA和GA串扰的机制在第4章进行了报道。与ABA类似， SA可能通过抑制α-淀粉酶基因（例如Amy32b）的表达来阻止种子发芽。糊粉细胞中SA诱导型和GA抑制型HvWRKY38的过度表达可阻断GA诱导的Amy32b表达。因此，HvWRKY38可能在调节α-淀粉酶表达和种子萌发中介导SA，ABA和GA信号的串扰。；我的研究证明了将生物信息学和实验生物学相结合的方法在解决细胞生物学信号网络方面的高效性。这项研究得出的发现有助于实现我们的长期目标：提高稻米和其他谷物的产量和质量。

著录项

  • 作者

    Xie, Zhen.;

  • 作者单位

    University of Nevada, Las Vegas.;

  • 授予单位 University of Nevada, Las Vegas.;
  • 学科 Biology Molecular.;Biology Cell.;Biology Bioinformatics.;Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 186 p.
  • 总页数 186
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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