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Correlation of physicochemical properties of chondroitin sulfate to its in-vitro absorption and anti-inflammatory activity.

机译：硫酸软骨素的理化性质与其体外吸收和抗炎活性的关系。

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摘要

The purpose of this study was to investigate if the molecular weight and degree of sulfation of chondroitin sulfate (CS) has an impact on its in vitro absorption and anti-inflammatory activity.;For absorption studies, Caco-2 cells were incubated with eight CS samples of differing molecular weights (7 kDa - 35 kDa). The amount of CS transported into the basolateral side of the Caco-2 monolayer was quantitatively determined to calculate the permeability coefficients (Peff). The permeability coefficients of the eight different CS samples across Caco-2 cell monolayers were assessed. For anti-inflammatory studies, RAW 264.7 murine macrophage cells were pre incubated with the CS samples for an hour followed by addition of bacterial lipopolysaccharide (LPS). The anti-inflammatory activity of CS samples was measured by the ability of CS samples to inhibit expression of a panel of inflammatory cytokines- tumor necrosis factor-alpha (TNF-α), Interleukin-1 beta (IL-1βand Interleukin-6 (IL-6). These inflammatory markers were quantitatively measured using ELISA and inhibition of nitric oxide (NO) production was measured using Griess reagent assay.;Of the eight samples evaluated, four had a Peff value of 15 x 10-6 cm sec-1 or higher indicating moderate to high absorption. Two of the four samples with higher Peff values were high molecular weight compounds (∼ 35 kDa). At concentrations of 5 μg/ml as well as 15 μg/ml, CS samples significantly inhibited expression of LPS induced TNF-α. Expression of IL-6 was inhibited by some of the CS samples at 15 μg/ml concentration but not at 5μg/ml. Under the experimental conditions, IL-1β and NO were not useful in estimating the anti-inflammatory activity of the CS samples. Statistical analysis which examined the relation between molecular weight and each of these inflammatory markers revealed no correlation (p<0.05).;Within the CS molecular weight range used in this experiment, the absorption of CS samples did not have a correlation with their molecular weights but, interestingly, correlation was observed between the absorption and percentage of 6-sulfated disaccharide in the CS samples. CS samples used in this study appeared to inhibit some of the inflammatory cytokines but no correlation seemed to exist between the molecular weights and anti-inflammatory activity of these samples.
机译：这项研究的目的是研究硫酸软骨素（CS）的分子量和硫酸化程度是否对其体外吸收和抗炎活性产生影响。;为了进行吸收研究，将Caco-2细胞与8种CS一起孵育分子量不同（7 kDa-35 kDa）的样品。定量确定运到Caco-2单层基底外侧的CS的量，以计算渗透系数（Peff）。评估了八个不同CS样品在Caco-2细胞单层上的渗透系数。为了进行抗炎研究，将RAW 264.7鼠巨噬细胞与CS样品预孵育一个小时，然后添加细菌脂多糖（LPS）。通过CS样品抑制一组炎症细胞因子-肿瘤坏死因子-α（TNF-α），IL-1β（IL-1β和IL-6（IL）的表达的能力来测量CS样品的抗炎活性-6）。使用ELISA定量测量了这些炎症标记，使用Griess试剂测定法测量了对一氧化氮（NO）的抑制。 Peff值较高的四个样品中有两个是高分子量化合物（约35 kDa），在5μg/ ml和15μg/ ml的浓度下，CS样品均显着抑制LPS的表达在某些浓度为15μg/ ml的CS样品中抑制了IL-6的表达，而在5μg/ ml的浓度下没有抑制IL-6的表达；在实验条件下，IL-1β和NO不能用于评估抗炎性CS样品的活性。统计分析分子量与这些炎症标记物之间的关系没有相关性（p <0.05）。；在本实验使用的CS分子量范围内，CS样品的吸收与其分子量没有相关性，但有趣的是，在CS样品中观察到6硫酸化二糖的吸收率和百分比之间存在相关性。在这项研究中使用的CS样品似乎可以抑制某些炎症细胞因子，但这些样品的分子量和抗炎活性之间似乎没有相关性。

著录项

  • 作者

    Surapaneni, Lahari.;

  • 作者单位

    Clemson University.;

  • 授予单位 Clemson University.;
  • 学科 Agriculture Food Science and Technology.;Health Sciences Nutrition.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 107 p.
  • 总页数 107
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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