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UV-visible microscope spectrophotometric polarization and dichroism with increased discrimination power in forensic analysis.

机译：紫外可见分光光度法偏振和二色性在法医分析中具有更高的鉴别能力。

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摘要

Microanalysis of transfer (Trace) evidence is the application of a microscope and microscopical techniques for the collection, observation, documentation, examination, identification, and discrimination of micrometer sized particles or domains. Microscope spectrophotometry is the union of microscopy and spectroscopy for microanalysis. Analytical microspectroscopy is the science of studying the emission, reflection, transmission, and absorption of electromagnetic radiation to determine the structure or chemical composition of microscopic-size materials. Microscope spectrophotometry instrument designs have evolved from monochromatic illumination which transmitted through the microscope and sample and then is detected by a photometer detector (photomultiplier tube) to systems in which broad-band (white light) illumination falls incident upon a sample followed by a non-scanning grating spectrometer equipped with a solid-state multi-element detector. Most of these small modern spectrometers are configured with either silicon based charged-couple device detectors (200-950 nm) or InGaAs based diode array detectors (850-2300 nm) with computerized data acquisition and signal processing being common.;A focus of this research was to evaluate the performance characteristics of various modern forensic (UV-Vis) microscope photometer systems as well as review early model instrumental designs. An important focus of this research was to efficiently measure ultraviolet-visible spectra of microscopically small specimens for classification, differentiation, and possibly individualization. The first stage of the project consisted of the preparation of microscope slides containing neutral density filter reference materials, molecular fluorescence reference materials, and dichroic reference materials. Upon completion of these standard slide preparations analysis began with measurements in order to evaluate figures of merit for comparison of the instruments investigated. The figures of merit investigated included: 1) wavelength accuracy, 2) wavelength precision, 3) wavelength resolution stability, 4) photometric accuracy, 5) photometric precision, 6) photometric linearity, 7) photometric noise, and 8) short-term baseline stability. In addition, intrinsic instrument polarization effects were investigated to determine the impact of these properties on spectral interpretation and data quality.;Finally, a set of recommendations were developed which describe instrument performance characteristics for microscope and spectrometer features and functions, and specific instrument parameters that must be controlled in order to acquire high quality data from an ultraviolet-visible forensic microscope spectrophotometer system for increased discrimination power.
机译：转移的微观分析（痕迹）证据是显微镜和显微技术的应用，用于收集，观察，记录，检查，鉴定和区分微米尺寸的颗粒或区域。显微镜分光光度法是显微分析与光谱学的结合。分析显微光谱学是研究电磁辐射的发射，反射，透射和吸收以确定微观尺寸材料的结构或化学组成的科学。显微镜分光光度仪的设计已经从单色光（通过显微镜和样品传输），然后由光度计检测器（光电倍增管）进行检测，演变成宽带（白光）照明入射到样品，然后再入射到非光谱系统的系统。扫描光栅光谱仪配备了固态多元素检测器。这些小型现代光谱仪中的大多数都配置了硅基电荷耦合器件检测器（200-950 nm）或InGaAs基二极管阵列检测器（850-2300 nm），它们通常具有计算机化的数据采集和信号处理功能。研究旨在评估各种现代法医（UV-Vis）显微镜光度计系统的性能特征，并审查早期模型仪器设计。这项研究的重要重点是有效测量微观小样本的紫外可见光谱，以进行分类，区分和可能的个性化。该项目的第一阶段包括准备载有中性密度滤光片参考材料，分子荧光参考材料和二向色性参考材料的显微镜载玻片。这些标准载玻片制备完成后，分析开始于测量，以评估品质因数以比较所研究的仪器。研究的品质因数包括：1）波长精度，2）波长精度，3）波长分辨率稳定性，4）光度精度，5）光度精度，6）光度线性，7）光度噪声和8）短期基线稳定性。此外，还研究了仪器固有的偏振效应，以确定这些特性对光谱解释和数据质量的影响。最后，提出了一系列建议，描述了显微镜和光谱仪特征和功能的仪器性能特征以及特定的仪器参数，必须对其进行控制，以便从紫外可见的法医显微镜分光光度计系统获取高质量的数据，以提高辨别能力。

著录项

  • 作者

    Purcell, Dale Kevin.;

  • 作者单位

    City University of New York.;

  • 授予单位 City University of New York.;
  • 学科 Philosophy of Science.;Chemistry Analytical.;Physics Optics.;Chemistry General.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 278 p.
  • 总页数 278
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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