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Muscle growth and gene expression in the Pacific white shrimp Litopenaeus vannamei over the molt cycle.

机译：整个太平洋蜕皮虾南美白对虾在蜕皮周期中的肌肉生长和基因表达。

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摘要

This study was designed to investigate molt-induced responses affecting muscle growth in the pacific white shrimp Litopenaeus vannamei. Cellular, molecular and genetic analyses were carried out in abdominal muscle. Clear images of uropod setogenesis and muscle myofibers were presented. Water, total soluble proteins, DNA, and RNA levels presented specific variations during the molt cycle. A reverse transcriptase multiplex PCR method for semi-quantitative detection of mRNA from muscle genes was developed. Five genes (muscle actin, beta-actin, myosin heavy chain, ubiquitin and heat shock protein 70) were studied and mRNA levels were normalized to an internal control housekeeping gene. Results confirmed that there are specific regulations of gene expressions during molt cycle. A shrimp abdominal muscle cDNA library was constructed and screened to investigate the diversity of expressed sequence tags and to identify new sequences that may play a role in regulation of muscle growth. Direct sequence of 311 randomly picked clones revealed 109 sequences with high homology (blastn score >100 bits) to annotated sequences from DNA databases. Homologous genes were grouped in 8 major functional categories. Sixty three sequences were considered unknowns. Contig creation grouped 77 sequences in 37 contigs with 430nt of average length of insert. These results illustrate the diversity of genes expressed in shrimp muscle, and also revealed that EST analysis is a suitable methodology for gene discovery in shrimps. Overall results of this project enhanced our understanding of muscle growth in shrimp. As shrimp aquaculture is becoming an important sector of world production of seafood, the knowledge generated by this work supports further studies that will favor genetic selection of fast growing shrimp lines, which can decrease growout cycles, productions costs, and reduce the time of exposure to diseases.
机译：这项研究旨在调查蜕皮诱发的反应，影响太平洋南美白对虾凡纳滨对虾的肌肉生长。在腹部肌肉中进行细胞，分子和遗传分析。呈现了足节定殖和肌肉肌纤维的清晰图像。在蜕皮周期中，水，总可溶性蛋白质，DNA和RNA含量呈现出特定的变化。建立了一种反转录酶多重PCR方法，用于从肌肉基因中半定量检测mRNA。研究了五个基因（肌动蛋白，β-肌动蛋白，肌球蛋白重链，遍在蛋白和热休克蛋白70），并将mRNA水平相对于内部对照管家基因进行了标准化。结果证实在蜕皮周期中基因表达有特定的规定。构建并筛选了虾腹肌cDNA文库，以研究表达的序列标签的多样性并鉴定可能在调节肌肉生长中发挥作用的新序列。 311个随机挑选的克隆的直接序列揭示了109个与DNA数据库注释序列具有高度同源性（blastn得分> 100位）的序列。同源基因分为8个主要功能类别。 63个序列被认为是未知序列。重叠群的创建将37个重叠群中的77个序列分组，平均插入长度为430nt。这些结果说明了在虾肌肉中表达的基因的多样性，并且还表明EST分析是在虾中发现基因的合适方法。该项目的总体结果增进了我们对虾肌肉生长的了解。随着虾类水产养殖业成为世界海产品生产的重要部门，这项工作所产生的知识为进一步的研究提供了支持，这些研究将有利于对快速生长的虾系进行基因选择，这可以减少成虾周期，生产成本并减少接触海藻的时间。疾病。

著录项

  • 作者

    Cesar, Jose Renato de Oliveria.;

  • 作者单位

    University of Hawai'i at Manoa.;

  • 授予单位 University of Hawai'i at Manoa.;
  • 学科 Biology Molecular.; Biology Zoology.; Agriculture Fisheries and Aquaculture.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 96 p.
  • 总页数 96
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;动物学;水产、渔业;
  • 关键词

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