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首页> 外文学位 >Molecular properties of Texas Phoenix decline phytoplasma, a subgroup 16SrIV-D strain associated with lethal diseases of Sabal palmetto and other palms in Florida.
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Molecular properties of Texas Phoenix decline phytoplasma, a subgroup 16SrIV-D strain associated with lethal diseases of Sabal palmetto and other palms in Florida.

机译：Texas Phoenix的分子特性下降了植物质体，这是与佛罗里达州的Sabal palmetto和其他棕榈的致死性疾病相关的16SrIV-D亚型菌株。

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摘要

A decline of sabal or cabbage palm (Sabal palmetto) with symptoms similar to those of coconut (Cocos nucifera) lethal yellowing (LY) was first observed in west central Florida, USA in 2008. Characterization of the causal agent implicated a phytoplasma, which closely resembled a subgroup 16SrIV-D phytoplasma previously associated with declining Canary Island date palms (Phoenix canariensis), edible date (P. dactylifera), silver date (P. sylvestris) and Queen (Syagrus romanzoffiana) palms. The phytoplasma strain associated with the Florida native S. palmetto was therefore also referred to as Texas Phoenix decline (TPD) phytoplasma, and classified as a subgroup 16SrIV-D strain. Although this phytoplasma had been classified based on the 16S rRNA sequence, further characterization of its genome remained crucial. Twenty five S. palmetto plants in west central Florida showing symptoms of decline were selected for sampling of the phytoplasma associated with the disease. DNA was extracted from tissue samples and the intergenic spacer region between 16S rRNA and 23S rRNA genes was amplified by a nested polymerase chain reaction assay. The sequence of the 16S-23S intergenic spacer (IGS) of TPD phytoplasma infecting S. palmetto was compared with IGS sequences derived from six 16SrIV-D phytoplasma strains infecting Phoenix spp., a 16SrIV-D strain infecting Syagrus romanzoffiana, a 16SrIV-F strain infecting Washingtonia robusta, two strains each comprising a mixture of 16SrIV-A and 16SrIV-F phytoplasmas, one LY phytoplasma strain from P. canariensis and finally those of three 16SrIV-A strains associated with LY disease of C. nucifera. The TPD phytoplasma population associated with S. palmetto appears homogenous. On the basis of the ribosomal operon, the TPD phytoplasma in S. palmetto is similar to other TPD strains found in Phoenix spp. and S. romanzoffiana, but different from the subgroup 16SrIV-A phytoplasma associated with C. nucifera and Phoenix spp. For further analysis, three genes were selected: the nusA gene, the hflB gene, and the gcp gene. All genes (nusA, hflB and gcp) differentiated the strains in this study similarly, TPD phytoplasma strain infecting S. palmetto is similar to the TPD strain infecting Cocos and Phoenix spp. but different from the LY strains associated with C. nucifera.
机译：2008年在美国佛罗里达州中西部首次观察到sa蒲或卷心菜棕榈（Sabal palmetto）下降的症状类似于椰子（Cocos nucifera）致死性黄变（LY）。致病因子的特性与植物原浆有关，其紧密相关类似于先前与下降的加那利岛枣椰子（Phoenix canariensis），可食用枣（P. dactylifera），银枣（P. sylvestris）和皇后（Syagrus romanzoffiana）棕榈相关的16SrIV-D亚种亚群。因此，与佛罗里达州天然棕榈链霉菌相关的植物原质菌株也被称为德克萨斯凤凰下降（TPD）植物原质，并被分类为16SrIV-D亚型。尽管已经根据16S rRNA序列对该植物质体进行了分类，但对其基因组的进一步表征仍然至关重要。选择了佛罗里达州中西部有25种表现出下降症状的棕榈糖链霉菌（S. palmetto）植物来取样与该病有关的植物原质。从组织样品中提取DNA，并通过巢式聚合酶链反应法扩增16S rRNA和23S rRNA基因之间的基因间隔区。比较了感染棕榈棕榈链霉菌的TPD植物质体的16S-23S基因间隔区（IGS）的序列与来源于感染Phoenix spp。的16SrIV-D植物质体的6种16SrIV-D菌株的IGS序列的比较，该16SrIV-D菌株感染了Sygrus romanzoffiana，一种16SrIV-F该菌株感染了强壮的华盛顿菌，两个菌株分别包含16SrIV-A和16SrIV-F植物质原体的混合物，一个来自加拿大假单胞菌的LY植物质体，最后是三个与C. nucifera LY疾病相关的16SrIV-A菌株。与棕榈棕榈链霉菌相关的TPD植物胞质种群似乎是同质的。根据核糖体操纵子，棕榈链霉菌中的TPD植物质浆与Phoenix spp中发现的其他TPD菌株相似。和S. romanzoffiana，但不同于亚群C. nucifera和Phoenix spp的16SrIV-A亚种质体。为了进一步分析，选择了三个基因：nusA基因，hflB基因和gcp基因。在本研究中，所有基因（nusA，hflB和gcp）均对菌株进行了区分，感染S. palmetto的TPD胞质菌株类似于感染Cocos和Phoenix spp的TPD菌株。但不同于与C. nucifera相关的LY菌株。

著录项

  • 作者

    Ntushelo, Khayalethu.;

  • 作者单位

    University of Florida.;

  • 授予单位 University of Florida.;
  • 学科 Biology Molecular.;Agriculture Plant Culture.;Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 85 p.
  • 总页数 85
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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