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首页> 外文学位 >In vitro studies on the role of seminal plasma proteins on the interaction between polymorphonuclear neutrophils (PMNs) and spermatozoa in the horse.
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In vitro studies on the role of seminal plasma proteins on the interaction between polymorphonuclear neutrophils (PMNs) and spermatozoa in the horse.

机译：体外研究精浆蛋白对马中多形核中性粒细胞（PMN）与精子之间相互作用的作用。

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摘要

Semen induces a transient uterine inflammatory response in the mare, characterized by an influx of polymorphonuclear neutrophils (PMNs). The physiological role of this inflammation is to clear excess and damaged spermatozoa and bacterial contaminants from the uterus in order to provide an environment that is compatible with normal embryo development and survival. It has been shown that seminal plasma protects spermatozoa from being phagocytosed and destroyed in an inflammatory environment. In addition, previous work has identified the protein fraction in seminal plasma as the component responsible for this effect. The objectives of these studies were to identify and characterize specific seminal plasma protein(s) that are involved in the protection of spermatozoa from PMN binding, and to examine the binding capacity of PMNs when exposed to live or dead spermatozoa and bacteria in the presence or absence of this protein. Secondarily, we examined the interaction between PMNs and spermatozoa in real time. The identity of the protective protein was discovered to be CRISP-3. This protein protects live spermatozoa from binding but did not protect dead spermatozoa or bacteria. Polymorphonuclear neutrophils also exhibit a secondary removal mechanism for dead or damaged sperm cells, through the formation of extracellular traps (NETs). This is the first time a biological function has been described for CRISP-3 in seminal plasma. It is also the first time a selective mechanism in the uterus has been demonstrated and that CRISP-3 functions to protect live sperm cells only.
机译：精液在母马中引起短暂的子宫炎症反应，其特征是多形核中性粒细胞（PMN）大量涌入。这种炎症的生理学作用是从子宫清除过量和受损的精子和细菌污染物，以提供与正常胚胎发育和存活相适应的环境。已经显示出精浆能保护精子免于在炎性环境中被吞噬和破坏。此外，先前的工作已经确定了精浆中的蛋白质部分是造成这种效应的原因。这些研究的目的是鉴定和鉴定参与保护精子免受PMN结合的特定精浆血浆蛋白，并检查暴露于活的或死的精子和细菌时，PMN的结合能力。这种蛋白质的缺乏。其次，我们实时检查了PMN与精子之间的相互作用。发现保护蛋白的身份是CRISP-3。这种蛋白质可以保护活的精子免受结合，但不能保护死的精子或细菌。多形核中性粒细胞还通过形成细胞外捕集器（NET）表现出对死亡或受损的精子细胞的二级清除机制。这是首次在精浆中描述CRISP-3的生物学功能。这也是首次证明子宫中的选择性机制，CRISP-3仅起到保护活精子细胞的作用。

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  • 作者

    Doty, Andria L.;

  • 作者单位

    University of Florida.;

  • 授予单位 University of Florida.;
  • 学科 Biology Physiology.;Health Sciences Immunology.;Agriculture Animal Culture and Nutrition.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 177 p.
  • 总页数 177
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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