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A comparative study of three methods to enhance the collection of DNA from plant material.

机译：三种增强植物材料DNA收集方法的比较研究。

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摘要

The Botanical Research Institute of Texas is using two methods of DNA extraction from plants, an automated method called Bullet BlenderRTM and a manual method of grinding. A third method, using an instrument called the Fast Prep-24(TM), was evaluated and the DNA yield obtained was compared to the other methods. Eight plant species were chosen and two sample preparation methods, wet and dry, were evaluated. DNA yield gels were run in order to compare DNA quality and UV spectroscopy was used to evaluate quantity. Independent Student t-tests were performed to compare means variation between the DNA yield on the wet and dry samples and one-way ANOVA was used to compare variation between the three extraction methods. No significant difference was found between the wet and dry samples for DNA concentrations, but a significant difference was observed between the Fast Prep-24(TM) instrument and the other two methods.
机译：德克萨斯州植物研究所正在使用两种从植物中提取DNA的方法，一种称为Bullet BlenderRTM的自动化方法，另一种是手动研磨方法。使用称为Fast Prep-24 TM的仪器评估了第三种方法，并将获得的DNA产量与其他方法进行了比较。选择了八种植物，并评估了湿法和干法两种样品制备方法。运行DNA产量凝胶以比较DNA质量，并使用UV光谱法评估数量。进行了独立的学生t检验，以比较湿样品和干样品上DNA产量之间的均值差异，并使用单向ANOVA来比较三种提取方法之间的差异。湿样品和干样品之间的DNA浓度没有发现显着差异，但是Fast Prep-24™仪器与其他两种方法之间却观察到显着差异。

著录项

  • 作者

    Ausmer, Alea.;

  • 作者单位

    University of North Texas Health Science Center at Fort Worth.;

  • 授予单位 University of North Texas Health Science Center at Fort Worth.;
  • 学科 Biology Botany.
  • 学位 M.S.
  • 年度 2013
  • 页码 42 p.
  • 总页数 42
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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