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首页> 外文学位 >Genetics of Seed Coat and Stem Development in Flax ( Linum usitatissimum L.).
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Genetics of Seed Coat and Stem Development in Flax ( Linum usitatissimum L.).

机译：亚麻（Linum usitatissimum L.）种子皮和茎发育的遗传。

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摘要

Flax (linseed, Linum usitatissimum L) is cultivated either for its seeds or its stem (bast fiber). With the goal of contributing to development of novel flax germplasm, a forward genetics screen of EMS mutagenized flax (var. CDC Bethune) was conducted to identify seeds with abnormal pigmentation or mucilage. Seeds of 16,764 M4 families were examined visually, with and without staining by Toluidine Blue. A total of 86 putative seed color mutants were identified, with the most common phenotype being yellow seeds. There were 1,975 identified putative mucilage mutants, with a range of phenotypes, including Blue Seed, No Mucilage Released, and Abnormal Mucilage Released. A subset of the seed colors and mucilage phenotypes were tested for heritability. The seed colors were generally heritable, but the mucilage phenotypes appeared to show variable expressivity that is probably highly dependent on the environment. Histological analysis revealed how seed coat anatomy changes affected seed color, but did not explain the mucilage phenotypes tested. The lines have been provided to flax breeders other collaborators for further analysis and potential use in breeding programs.;GASA (Gibberellic Acid Stimulated in Arabidopsis) genes encode a family of small proteins of unknown biochemical function, whose expression has been correlated with various processes in plants including hormone response, defense, and development. GASA genes have also been reported in our lab to be expressed in developing flax stems. To better characterize the GASA-like gene family in flax, I identified 20 predicted GASA-like genes in the flax whole genome assembly. Using microarray and qRT-PCR analysis, seven of these genes showed differential transcript expression during stem development. I cloned and verified the sequences of these genes. Based on these studies, I was able to define groups of flax GASA-like genes whose expression was well-correlated with stem segments in either the elongation or cell wall deposition stage. These results provide further evidence that specific GASA genes are involved in different processes in flax stem and/or fiber development.
机译：亚麻（亚麻籽，亚麻）的种植是为了获得其种子或茎（韧皮纤维）。为了促进新型亚麻种质的发展，对EMS诱变亚麻（var。CDC Bethune）进行了正向遗传学筛选，以鉴定色素沉着或粘液异常的种子。目视检查有和没有甲苯胺蓝染色的16,764个M4家族的种子。总共鉴定出86种种子颜色突变体，最常见的表型是黄色种子。鉴定出了1,975个推定的粘液突变体，其表型范围包括蓝种子，无粘液释放和异常粘液释放。测试了种子颜色和黏液表型的子集的遗传力。种子的颜色通常是可遗传的，但粘液表型似乎表现出可变的表达能力，这可能与环境高度相关。组织学分析显示种皮解剖结构变化如何影响种子颜色，但没有解释所测试的粘液表型。该品系已提供给亚麻育种者其他合作者，以供进一步分析和在育种计划中的潜在用途。GASA（拟南芥刺激的赤霉素）基因编码一类生化功能未知的小蛋白家族，其表达与大肠杆菌中的各种过程相关植物，包括激素反应，防御和发育。在我们的实验室中也有报道称GASA基因会在发育中的亚麻茎中表达。为了更好地表征亚麻中的GASA样基因家族，我在亚麻全基因组装配中鉴定了20个预测的GASA样基因。使用微阵列和qRT-PCR分析，这些基因中的七个在茎发育过程中表现出差异的转录表达。我克隆并验证了这些基因的序列。基于这些研究，我能够确定亚麻GASA样基因的组，这些基因的表达在伸长或细胞壁沉积阶段与茎节紧密相关。这些结果提供了进一步的证据，表明特定的GASA基因参与了亚麻茎和/或纤维发育的不同过程。

著录项

  • 作者

    To, Lai Thi.;

  • 作者单位

    University of Alberta (Canada).;

  • 授予单位 University of Alberta (Canada).;
  • 学科 Biology Botany.;Biology Genetics.
  • 学位 M.S.
  • 年度 2013
  • 页码 120 p.
  • 总页数 120
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 老年病学;
  • 关键词

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