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首页> 外文学位 >NMR studies of myoglobin translational diffusion in perfused myocardium and conformational analysis on the poly-gamma-D-glutamic acid capsule of Bacillus licheniformis: Implication on the regulation of oxygen transport and a virulence factor in anthrax.
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NMR studies of myoglobin translational diffusion in perfused myocardium and conformational analysis on the poly-gamma-D-glutamic acid capsule of Bacillus licheniformis: Implication on the regulation of oxygen transport and a virulence factor in anthrax.

机译：地衣芽孢杆菌聚-γ-D-谷氨酸胶囊中肌红蛋白翻译扩散在心肌中的NMR研究和构象分析：对炭疽中氧转运和毒力因子的调控意义。

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摘要

NMR has become one of the most important techniques to study dynamic and static protein structures as well as physiological and clinical researches. In this dissertation the main focuses have been to investigate the translational diffusion behavior of myoglobin (Mb) in myocardium and to explore the conformation of the capsular poly-gamma-D-glutamate (gamma-D-PGA) by means of NMR spectroscopy.; Due to the capability of observing the distinct Val E11 gamma-methyl group signal of oxymyoglobin in vivo, the pulsed-field gradient NMR methods have been applied to determine the endogenous Mb translational diffusivity in perfused rat myocardium for the first half of the dissertation. The results demonstrate that the averaged translational diffusion coefficient of the endogenous Mb has a value of 4.24 x 10-7 cm 2/s at 22°C and it rises up to 8.37 x 10-7 cm2/s at 40°C. No orientation preference of Mb diffusivity has been observed within this temperature range, corresponding to a mean displacement of 2.5--3.5 mum. Given the resting PO2 in respiring muscle in vivo of around 10 mmHg, Mb has no general role in facilitated O2 transport under basal physiological condition.; The conformational change of the gamma-D-PGA synthesized by Bacillus licheniformis ATTC 9945A has been investigated by 1H NMR under various conditions for the second part of this dissertation. The experimental data present that the gamma-D-PGA endures a mixed secondary structure of the coil and helix forms. In particular, the results in this study do not reveal obvious conformational change of the purified gamma-D-PGA induced by pH because only do the minor differences in the chemical shifts show on 1H NMR spectra. Owning to small quantities of paramagnetic ion contamination in this biopolymer aqueous solution, the formation of a H2O-ion-gamma-D-PGA complex is affected by different pH and ion-strength environments, performing on the 1H NMR spectra and relaxation times.; In summary, the Mb translational diffusion determined in this study has then helped to clarify the role of Mb in facilitating O2 transport in vivo; it also reveals the effect of paramagnetic ions on the conformation of gamma-D-PGA.
机译：NMR已成为研究动态和静态蛋白质结构以及生理和临床研究的最重要技术之一。本论文的主要研究重点是通过核磁共振波谱法研究肌红蛋白（Mb）在心肌中的翻译扩散行为，并探索荚膜聚γ-D-谷氨酸（γ-D-PGA）的构象。由于能够观察到体内氧合肌红蛋白独特的Val E11γ-甲基信号，因此在本文的上半部分，采用脉冲场梯度NMR方法确定了灌注大鼠心肌中的内源Mb转化扩散率。结果表明，内源性Mb的平均平移扩散系数在22°C时为4.24 x 10-7 cm 2 / s，在40°C时上升至8.37 x 10-7 cm2 / s。在此温度范围内未观察到Mb扩散性的取向偏向，相当于2.5--3.5微米的平均位移。鉴于体内呼吸肌肉中的静息PO2约为10 mmHg，Mb在基础生理条件下在促进O2转运方面没有一般作用。本文的第二部分通过1 H NMR在多种条件下研究了地衣芽孢杆菌ATTC 9945A合成的γ-D-PGA的构象变化。实验数据表明，γ-D-PGA可以承受线圈和螺旋形式的混合二级结构。特别是，这项研究的结果并未揭示由pH诱导的纯化的γ-D-PGA的明显构象变化，因为在1H NMR光谱上仅显示出化学位移的微小差异。由于该生物聚合物水溶液中存在少量顺磁性离子污染，H2O-离子-γ-D-PGA络合物的形成受1H NMR光谱和弛豫时间不同的pH和离子强度环境的影响。总之，本研究中确定的Mb翻译扩散有助于阐明Mb在促进体内O2转运中的作用。它也揭示了顺磁性离子对γ-D-PGA构象的影响。

著录项

  • 作者

    Lin, Ping-Chang.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Chemistry Polymer.; Biophysics Medical.; Biophysics General.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 161 p.
  • 总页数 161
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 高分子化学（高聚物）;生物物理学;生物物理学;
  • 关键词

  • 入库时间 2022-08-17 11:40:53

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