• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Differential expression and detection of transcripts in sweetpotato (Ipomoea batatas (L.) Lam.) using cDNA microarrays.
【24h】

Differential expression and detection of transcripts in sweetpotato (Ipomoea batatas (L.) Lam.) using cDNA microarrays.

机译：利用cDNA微阵列在甘薯（Ipomoea batatas（L.）Lam。）中差异表达和检测转录本。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microarray protocols were developed for sweetpotato (Ipomoea batatas (L.) Lam.) and then used to study issues of importance in sweetpotato physiology and production. The effect of replication number and image analysis software was compared with results obtained by quantitative real-time PCR. The results indicated that reliable results could be obtained using six replicates and UCSF Spot image analysis software. These methodologies were employed to elucidate aspects of sweetpotato development, physiology and response to virus infection. Storage root formation is the most economically important process in sweetpotato development. Gene expression levels were compared between fibrous and storage roots of the cultivar Jewel. Sucrose synthase, ADP-glucose pyrophosphorylase, and fructokinase were up-regulated in storage roots, while hexokinase was not differentially expressed. A variety of transcription factors were differentially expressed as well as several auxin-related genes. The orange flesh color of sweetpotato is due to beta-carotene stored in chromoplasts of root cells. beta-carotene is important because of its role in human health. To elucidate biosynthesis and storage of beta-carotene in sweetpotato roots, microarray analysis was used to investigate genes differentially expressed between 'White Jewel' and 'Jewel' storage roots. beta-carotene content calculated for 'Jewel' and 'White Jewel' were 20.66 mg/100 g fresh weight (FW) and 1.68 mg/100 g FW, respectively. Isopentenyl diphosphate isomerase was down-regulated in 'White Jewel', but three other genes in the beta-carotene biosynthetic pathway were not differentially expressed. Several genes associated with chloroplasts were differentially expressed, indicating probable differences in chromoplast development of 'White Jewel' and 'Jewel'. Sweet potato virus disease (SPVD) is caused by the co-infection of plants with a potyvirus, Sweet potato feathery mottle virus (SPFMV), and a crinivirus, Sweet potato chlorotic stunt virus (SPCSV). Expression analysis revealed that the number of differentially expressed genes in plants infected with SPFMV alone and SPCSV alone compared to virus-tested plants was only three and 14, respectively. In contrast, more than 200 genes from various functional categories were differentially expressed between virus-tested and SPVD-affected plants. Microarray analysis has proved to be a useful tool to study important aspects of sweetpotato physiology and production.
机译：开发了用于甘薯（Ipomoea batatas（L.）Lam。）的微阵列方案，然后用于研究在甘薯生理学和生产中的重要问题。将复制数量和图像分析软件的效果与通过定量实时PCR获得的结果进行了比较。结果表明，使用六个重复样品和UCSF Spot图像分析软件可以获得可靠的结果。这些方法被用来阐明甘薯发育，生理和对病毒感染的反应。贮藏根的形成是甘薯发展中最经济重要的过程。比较了品种Jewel的纤维根和贮藏根之间的基因表达水平。蔗糖合酶，ADP-葡萄糖焦磷酸化酶和果糖激酶在贮藏根中上调，而己糖激酶没有差异表达。多种转录因子以及几种生长素相关基因被差异表达。甘薯的橙色果肉是由于β-胡萝卜素储存在根细胞的质体中。 β-胡萝卜素很重要，因为它在人类健康中具有重要作用。为了阐明甜菜根中β-胡萝卜素的生物合成和贮藏，使用微阵列分析研究了“白色宝石”和“宝石”贮藏根之间差异表达的基因。计算出的“珠宝”和“白色珠宝”中的β-胡萝卜素含量分别为20.66 mg / 100 g新鲜体重（FW）和1.68 mg / 100 g FW。异戊烯基二磷酸异构酶在“白色宝石”中被下调，但β-胡萝卜素生物合成途径中的其他三个基因没有差异表达。与叶绿体有关的几个基因被差异表达，表明“白色宝石”和“珠宝”的色体发育可能存在差异。甘薯病毒病（SPVD）是由植物与波多病毒，甘薯羽状斑驳病毒（SPFMV）和crinivirus，甘薯褪绿特技病毒（SPCSV）共同感染引起的。表达分析表明，与仅用病毒测试的植物相比，仅用SPFMV和SPCSV感染的植物中差异表达基因的数量分别仅为3和14。相反，在经过病毒测试的植物和受SPVD影响的植物之间，有200多个来自各种功能类别的基因差异表达。芯片分析已被证明是研究甘薯生理和生产重要方面的有用工具。

著录项

  • 作者

    McGregor, Cecilia E.;

  • 作者单位

    Louisiana State University and Agricultural & Mechanical College.;

  • 授予单位 Louisiana State University and Agricultural & Mechanical College.;
  • 学科 Agriculture Plant Culture.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 143 p.
  • 总页数 143
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 作物生物学原理、栽培技术与方法;植物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号