• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >SNP-based Genetic Maps and Quantitative Trait Loci Controlling Bulb Quality in Segregating Haploid Progenies of Onion (Allium cepa L.).
【24h】

SNP-based Genetic Maps and Quantitative Trait Loci Controlling Bulb Quality in Segregating Haploid Progenies of Onion (Allium cepa L.).

机译：基于SNP的遗传图谱和定量鳞茎位点控制洋葱（Allium cepa L.）单倍体后代的鳞茎质量。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Onion (Allium cepa L.) is an important crop which possesses many health beneficial compounds, but also presents difficulties for genetic studies (i.e. complex genome, biennial generation, and inbreeding depression). To broaden the limited genomic resources, transcriptome sequencing using the Roche-454 platform of two inbred lines (OH1 and 5225) was performed. Single nucleotide polymorphisms (SNPs) were identified (3,364 on 1,716 cDNA contigs), and were mapped using gynogenic haploid families generated from F1 plants from the cross of OH1 and 5225. The most dense map of onion to date with 597 SNPs forming ten linkage groups was constructed from this study. Three hundred and thirty nine of these new SNPs also segregated in the previously developed F2 family from BYG15-23 x AC43. These two families shared 223 SNP markers, which were used to join maps and assign linkage groups to chromosomes. A second study characterized the genetics of red-bulb color and soluble-solids contents in the OH1 x 5225 haploid families. Quantitative trait loci were identified controlling the presence and intensity of anthocyanins in bulbs. Three QTLs, one on chromosome 7 and two in repulsion phase linkage on chromosome 4, conditioned the presence of anthocyanins. The quantities of the two anthocyanins [peonidin 3-glucoside and cyanidin 3-(6"-malonoyl-laminaribioside)] were assessed using high performance liquid chromatography (HPLC). Significant QTLs on chromosome 1 and 8 were associated with concentrations of peonidin 3-glucoside, while QTLs on chromosome 1 and 4 were associated with cyanidin 3-(6"-malonoyl-laminaribioside) concentrations. Significant correlations between the two anthocyanin concentrations and visual scores were detected. Consistent with previous studies, soluble-solids content 30-days postharvest was significantly associated with a region on chromosome 5. Genetic variation was discovered in yellow onion that contributed to increasing the intensity of red-bulb color in progenies.
机译：洋葱（洋葱Cepa L.）是一种重要的农作物，拥有许多对健康有益的化合物，但也给遗传研究（即复杂的基因组，每两年一次的繁殖和近亲衰退）带来了困难。为了拓宽有限的基因组资源，使用两个自交系（OH1和5225）的Roche-454平台进行了转录组测序。鉴定了单核苷酸多态性（SNP）（在1,716个cDNA重叠群上有3,364个），并使用从OH1和5225的杂交中从F1植物产生的雌核单倍体家族作图。迄今为止，最稠密的洋葱图具有597个SNP，形成了十个连接基团。是根据这项研究构建的。这些新的SNP中的139个也从BYG15-23 x AC43分离到了以前开发的F2家族中。这两个家族共有223个SNP标记，这些标记用于连接图谱和为染色体分配连锁组。第二项研究描述了OH1 x 5225单倍体家族中红鳞茎颜色和可溶性固形物含量的遗传。确定了定量性状基因座，以控制灯泡中花色苷的存在和强度。三个QTL（一个位于7号染色体上，两个处于Q4排斥相连锁状态）调节了花色苷的存在。用高效液相色谱法（HPLC）评估了两种花色苷的含量[peonidin 3-glucoside和cyanidin 3-（6“ -malonoyl-laminaribioside）]。1号和8号染色体上显着的QTL与peonidin 3-的浓度有关。葡萄糖苷，而染色体1和4上的QTL与花青素3-（6“-丙二酰-laminaribioside）浓度有关。检测到两个花色苷浓度和视觉评分之间存在显着相关性。与以前的研究一致，收获后30天的可溶性固形物含量与5号染色体上的一个区域显着相关。在黄洋葱中发现了遗传变异，这有助于增加子代红鳞茎的颜色强度。

著录项

  • 作者

    Duangjit, Janejira.;

  • 作者单位

    The University of Wisconsin - Madison.;

  • 授予单位 The University of Wisconsin - Madison.;
  • 学科 Biology Genetics.;Agriculture Horticulture.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 144 p.
  • 总页数 144
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号