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Molecular and functional studies of two Arabidopsis genes critical for microsporogenesis.

机译：对小孢子发生至关重要的两个拟南芥基因的分子和功能研究。

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摘要

The Arabidopsis solo dancers (sds ) mutant is sterile and defective in pollen production. Phenotypic characterization indicated that the SDS gene is required for normal synapsis, bivalent formation, and recombination in meiosis. Sequence analysis suggested that SDS encodes a putative cyclin. Considering that less information is known about plant cyclins than animal cyclins, I and collaborators first performed BLAST searches of available databases to obtain sequences of all cyclin genes in Arabidopis and in other vascular plants. We then defined ten classes of plant cyclins based on phylogenetic analyses. Expression analyses also showed that Arabidopsis cyclins exhibit diverse expression patterns at the tissue level. The results suggest that plants possess a larger and more complex family of cyclins than animals. To provide further evidence for SDS as a cyclin gene and characterize different SDS protein domains, I generated six different SDS truncations and studied them in the budding yeast. It was found that SDS can rescue a yeast G1 cyclin deficient mutant and can interfere with the mitotic cell cycle in wild type yeast. Comparative studies of the SDS truncations also suggested that a portion of the N-terminal region upstream of the cyclin domain is required for SDS function in the yeast system. Further study of the full-length SDS and an SDS truncation, SDS201-578, in Arabidopsis showed that the SDS N-terminal region is required for the full activity of SDS. The meiotic phenotypes in the partially rescued sds meiocytes by SDS201-578 confirmed the requirement of SDS in synapsis. Sequence alignment of SDS homologs showed that there are several highly conserved motifs in their N-terminal regions, suggesting a possible involvement of these motifs in SDS function.
机译：拟南芥独舞者（sds）突变体是不育的，并且在花粉生产中存在缺陷。表型特征表明SDS基因是减数分裂中正常突触，二价形成和重组所必需的。序列分析表明，SDS编码假定的细胞周期蛋白。考虑到有关植物细胞周期蛋白的信息少于动物细胞周期蛋白，我和合作者首先对可用数据库进行了BLAST搜索，以获得拟南芥和其他维管植物中所有细胞周期蛋白基因的序列。然后，根据系统发育分析，我们定义了十类植物细胞周期蛋白。表达分析还显示拟南芥细胞周期蛋白在组织水平上表现出多种表达模式。结果表明，植物比动物拥有更大，更复杂的细胞周期蛋白家族。为了提供SDS作为细胞周期蛋白基因并表征不同SDS蛋白结构域的进一步证据，我生成了6种不同的SDS截短图，并在发芽酵母中对其进行了研究。发现SDS可以拯救酵母G1细胞周期蛋白缺陷型突变体，并且可以干扰野生型酵母中的有丝分裂细胞周期。 SDS截短的比较研究还表明，在酵母系统中，SDS功能需要细胞周期蛋白域上游N末端区域的一部分。拟南芥对全长SDS和SDS截短SDS201-578的进一步研究表明，SDS的全部活性需要SDS N末端区域。通过SDS201-578在部分拯救的sds减数分裂细胞中的减数分裂表型证实了SDS在突触中的需求。 SDS同源物的序列比对显示，在其N端区域有几个高度保守的基序，表明这些基序可能与SDS功能有关。

著录项

  • 作者

    Wang, Guanfang.;

  • 作者单位

    The Pennsylvania State University.;

  • 授予单位 The Pennsylvania State University.;
  • 学科 Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 175 p.
  • 总页数 175
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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