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A quantitative study of chemically modified metallic surfaces for use in surgical implants.

机译：用于外科植入物的化学改性金属表面的定量研究。

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摘要

Titanium and its alloys, most notably Ti-6AI-4V, are widely used as surgical implants which are in contact with bone. Bonding the fibronectin cell attachment peptide, arginine-glycine-aspartic acid (RGD) to the native oxide surface of titanium might enhance its osteoconductivity by providing sites for the promotion of cell adhesion and spreading. Two surface modification procedures are described herein to immobilize RGD onto Ti-6Al-4V. The first method uses an o-terminated self-assembled monolayer (SAM) and an organic tether to provide a site for peptide attachment. The second method uses an organometallic, (tert-butoxy) zirconium surface complex for direct immobilization of the peptide via ligand metathesis with the tert-butoxy groups. Both methods are shown to produce a surface that is cell attractive; mouse fibroblast and human osteoblast cell culture studies indicate a significant increase over controls in the adhesion and spreading of cells, and in the laying down of focal points of adhesion by cells. The SAM based system is stable to hydrolysis, but the zirconium complex is cleaved from the surface over several days. Long-term studies show that cells begin the process of mineralization on the metal surface, which is necessary for the process of bone growth. In addition to its biocompatibility, this surface film also shows a high degree of interfacial strength against both shear and tensile stress.
机译：钛及其合金，最著名的是Ti-6AI-4V，被广泛用作与骨骼接触的外科植入物。将纤连蛋白细胞附着肽，精氨酸-甘氨酸-天冬氨酸（RGD）结合到钛的天然氧化物表面可能会通过提供促进细胞粘附和扩散的位点来增强其骨传导性。本文描述了两种将RGD固定在Ti-6Al-4V上的表面改性程序。第一种方法使用o端自组装单层（SAM）和有机系链来提供肽连接位点。第二种方法使用有机金属（叔丁氧基）锆表面复合物，通过配体复分解与叔丁氧基直接固定肽。两种方法均显示可产生具有细胞吸引力的表面。小鼠成纤维细胞和人成骨细胞的细胞培养研究表明，细胞的粘附和扩散以及细胞粘附点的放置都大大超过了对照。基于SAM的系统对水解稳定，但是锆配合物会在几天内从表面裂解。长期研究表明，细胞开始在金属表面上矿化，这是骨骼生长过程所必需的。除了其生物相容性外，该表面膜还显示出针对剪切应力和拉伸应力的高界面强度。

著录项

  • 作者

    Silverman, Brett Matthew.;

  • 作者单位

    Princeton University.;

  • 授予单位 Princeton University.;
  • 学科 Chemistry Inorganic.; Engineering Biomedical.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 129 p.
  • 总页数 129
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 无机化学;生物医学工程;
  • 关键词

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