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首页> 外文学位 >Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria.
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Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria.

机译：病原菌的蛋白质模式，蛋白质标记和整个蛋白质组的质谱和串联质谱表征。

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摘要

There have been many recent reviews published on MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight) MS (mass spectrometry) for identification of bacteria particularly with relevance to clinical microbiology. MALDI-TOF MS is now a mature technique for bacterial identification with great promise. The purpose of this review is to put into perspective MALDI-TOF MS and other widely used mass spectrometry methods for characterization of proteins. MALDI-TOF MS is used for rapid determination of a mass pattern of proteins for bacterial characterization; these proteins are generally not identified. Alternatively, after gel separation, MALDI TOF-TOF MSMS (tandem mass spectrometry) or on-line LC-ESI MS-MS (liquid chromatography electrospray tandem mass spectrometry) specific protein markers can be identified and peptide sequence variation among species assessed. Unlike direct MALDI-TOF MS, sample preparation for gel separation/MALDI-TOF-TOF MS and MS-MS remains quite demanding. Specific marker proteins are readily identified. Sample preparation is quite straightforward for LC-MS-MS. Massive amounts of information (whole proteomes) are provided but bioinformatics is complex. Chromatography and electrospray mass spectrometry instrumentation is also not widely used among microbiologists. Thus, there is a need for further development in sample preparation and instrumental development for rapid and simplified analysis. As MS-MS for microbial characterization reaches maturity, it is to be anticipated that further developments in bioinformatics will also become essential. The genome codes for all proteins that might be synthesized under certain growth conditions but only direct protein identification can prove that specific proteins or networks of proteins are actually expressed which might be of relevance in improving our understanding of bacterial pathogenesis.
机译：最近在MALDI-TOF MS（基质辅助激光解吸/电离飞行时间）MS（质谱）上发表了许多评论，以鉴定细菌，特别是与临床微生物有关的细菌。 MALDI-TOF MS现在是一种成熟的细菌鉴定技术，前景广阔。这篇综述的目的是透视MALDI-TOF MS和其他广泛使用的质谱法表征蛋白质的方法。 MALDI-TOF MS用于快速确定用于细菌鉴定的蛋白质质量模式；这些蛋白质通常无法鉴定。或者，在凝胶分离后，可以识别MALDI TOF-TOF MSMS（串联质谱）或在线LC-ESI MS-MS（液相色谱电喷雾串联质谱）特异性蛋白质标记，并评估物种之间的肽序列变异。与直接MALDI-TOF MS不同，用于凝胶分离/ MALDI-TOF-TOF MS和MS-MS的样品前处理仍然非常苛刻。特定的标记蛋白易于鉴定。对于LC-MS-MS，样品制备非常简单。提供了大量信息（整个蛋白质组学），但是生物信息学很复杂。色谱法和电喷雾质谱仪也没有在微生物学家中广泛使用。因此，需要进一步开发样品制备和仪器开发以进行快速和简化的分析。随着用于微生物表征的MS-MS的成熟，可以预见的是，生物信息学的进一步发展也将变得至关重要。基因组编码所有在特定生长条件下可能合成的蛋白质，但只有直接进行蛋白质鉴定才能证明特定蛋白质或蛋白质网络确实表达，这可能与增进我们对细菌发病机理的理解有关。

著录项

  • 作者

    Intelicato-Young, Jennifer.;

  • 作者单位

    University of South Carolina.;

  • 授予单位 University of South Carolina.;
  • 学科 Microbiology.
  • 学位 M.S.
  • 年度 2013
  • 页码 32 p.
  • 总页数 32
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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