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首页> 外文学位 >Effects of biocides on gene expression of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.
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Effects of biocides on gene expression of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.

机译：杀菌剂对硫酸盐还原细菌小球藻希尔登堡的基因表达的影响。

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摘要

Sulfate-reducing bacteria (SRB), such as i Hildenborough (DvH) cause unwanted souring (H2S formation) and corrosion in the oil and gas industry. Considerable efforts are made to eradicate SRB with biocides, such as glutaraldehyde (Glut), tetrakis (hydroxymethyl) phosphonium sulfate (THPS), and benzalkonium chloride (BAC). Although the response of DvH to environmental stressors such as oxygen, salt, and high temperature are well known, its responses to biocide stress have not been documented. Whole-genome microarrays of DvH treated with biocides, showed that 256, 96 and 198 genes were responsive to Glut, THPS, and BAC, respectively. Genes in energy production and conversion were induced by all three biocides. Genes in posttranslational modification, protein turnover and chaperones were induced the most by Glut, while genes in translation, ribosomal structure and biogenesis were induced the most by BAC. Many genes were unaffected by THPS. Constructed mutants for DVU0587-88 fdhAB, DVU0590, and DVU0035, which were up-regulated by Glut, had the same minimum inhibitory concentration and survival cell counts in the presence of Glut as the wild type. Interestingly, the DVU0590 and DVU0035 mutants grew slower in defined medium than the wild type. Because biocides affect many targets it may be difficult to achieve increased sensitivity by single gene mutations. Overall, this study provides the first insight of DvH gene expression response to biocides and has indicated that three commonly used biocides affect the physiology of the cell quite differently.
机译：Hildenborough（DvH）等硫酸盐还原细菌（SRB）会在石油和天然气工业中引起不良的酸味（H2S形成）和腐蚀。为了用诸如戊二醛（Glut），四（羟甲基）硫酸phospho（THPS）和苯扎氯铵（BAC）的杀生物剂消灭SRB，已经做出了巨大的努力。尽管DvH对诸如氧气，盐和高温之类的环境胁迫的响应是众所周知的，但尚未证明其对杀生物剂胁迫的响应。用杀生物剂处理的DvH的全基因组微阵列显示，分别有256、96和198个基因对Glut，THPS和BAC有反应。三种杀生物剂均诱导了能量产生和转化中的基因。 Glut诱导翻译后修饰，蛋白质更新和伴侣蛋白的基因最多，而BAC诱导翻译，核糖体结构和生物发生的基因最多。许多基因不受THPS的影响。由Glut上调的DVU0587-88 fdhAB，DVU0590和DVU0035的构建突变体在存在Glut的情况下具有与野生型相同的最小抑制浓度和存活细胞数。有趣的是，DVU0590和DVU0035突变体在特定培养基中的生长比野生型慢。由于杀生物剂影响许多靶标，可能难以通过单基因突变来提高敏感性。总的来说，这项研究提供了DvH基因表达对杀生物剂反应的第一见，并表明三种常用的杀生物剂对细胞生理的影响完全不同。

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  • 作者

    Lee, Meng-Hsin Phoebe.;

  • 作者单位

    University of Calgary (Canada).;

  • 授予单位 University of Calgary (Canada).;
  • 学科 Biology Molecular.;Biology Microbiology.;Biology Cell.
  • 学位 M.Sc.
  • 年度 2010
  • 页码 156 p.
  • 总页数 156
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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