• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >The Production of Hydrocarbons and other Lipids by Trichoderma koningii, Penicillium janthinellum and their Mixed Species Culture Grown Aerobically on Four Different Carbon Substrates.
【24h】

The Production of Hydrocarbons and other Lipids by Trichoderma koningii, Penicillium janthinellum and their Mixed Species Culture Grown Aerobically on Four Different Carbon Substrates.

机译：在四种不同碳底物上有氧生长的康宁木霉，黄花青霉及其混合菌种产生的烃类和其他脂质。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The fungal species, Trichoderma koningii, Penicillium janthinellum, and a mixture of Trichoderma koningii and Penicillium janthinellum were tested to determine their abilities to convert different carbon substrates (undecanoic acid (UDA), decanoic acid (DA), mixture of decanoic and undecanoic acid (UDA+DA), and mixture of potato dextrose broth and undecanoic acid (UDA+PDB) into different cellular metabolites. The mixed species culture showed higher biomass and carbon dioxide production on all 4 substrates compared to individual cultures. The mixed species culture showed highest biomass production of 59.5 mg/ml and cumulative carbon dioxide production of 394.2 mg after 576 hours of growth, when grown on mixture of potato dextrose broth and undecanoic acid. The intracellular accumulation of lipids in mixed species culture and single cultures was confirmed by fluorescent microscopy during stationary and exponential phase when UDA+PDB is source of carbon.;The analysis of fungal culture samples by pyrolysis-field ionization mass spectrometry (Py-FIMS) supported analysis by TLC-FID, and showed that fatty acid concentrations ranged between 4.4 and 6.6% of total molecular ion intensities (TIIs) depending on carbon substrate and ranged from n-C11 to n-C18. The Py-FIMS analysis shows that the intracellular hydrocarbons including n- alkanes, alkenes and n-alkyl esters were most abundant components with total yield ranging between 7.4 and 15.7% of total ion intensities (TIIs). When fungal cells were grown on fatty acids and PDB the C-chain lengths of extracellular alkanes ranged from n-C19 to n-C34, alkenes n-C30:1- nC40:1, and long chain alkyl esters from C41 - C60:1. According to Py-FIMS analyses, alkanes, alkenes and alkyl esters were not observed in a chloroform extract of PDB. The latter indicates that the studied mixed fungal species catalyze the conversion of fatty acids to hydrocarbons during growth.;Chemical analysis of different lipids produced by mixed species culture was analyzed by Iatroscan TLC-FID to identify 15 different lipid classes produced. The highest total intracellular lipid content of 3.5 mg/ml was observed when mixed species culture was grown on PDB+UDA during the exponential growth phase. Free fatty acids were the most dominant lipid class produced intracellularly and extracellularly during both the exponential and stationary growth phases. They account for >80% of total lipids produced. Analysis of intracellular and extracellular saturated and unsaturated fatty acids by GC-FID analysis shows, out of 55 fatty acids analyzed, the most dominating fatty acids on all substrates were palmitic acid (C16), stearic acid (C18 ), oleic acid (C18:1), and linoleic acid (C18:2), which account for nearly 70% of total free fatty acids (FFA) produced. The concentration of monounsaturated fatty acids and polyunsaturated fatty acids were similar, and their content ranged from 23% to 66% of the total FFA (0.007 to 0.57 mg/ml), and concentration of saturated fatty acids ranged between 33% and 75% of the total FFA (0.01 to 1.97 mg/ml) depending on substrate type and growth phase.
机译：测试了真菌物种konchoii的木霉菌，janthinellum的青霉菌和janthinellum的木霉菌的混合物，以确定它们转化不同碳底物（十一碳酸（UDA），癸酸（DA），癸酸和十一酸的混合物（ （UDA + DA），以及马铃薯葡萄糖肉汤和十一酸（UDA + PDB）混合成不同的细胞代谢产物，混合物种培养物在所有4种底物上的生物量和二氧化碳生成量均高于单独培养物在马铃薯葡萄糖肉汤和十一烷酸的混合物上生长576小时后，生物量产量为59.5 mg / ml，累积二氧化碳产量为394.2 mg。通过荧光显微镜确认混合物种培养和单一培养物中脂质的细胞内积累当UDA + PDB为碳源时，处于平稳和指数阶段。热解场电离质谱（Py-FIMS）支持的TLC-FID分析表明，脂肪酸浓度取决于总碳离子底物的总分子离子强度（TIIs）的4.4％至6.6％，范围从n-C11至n-C18。 Py-FIMS分析表明，胞内碳氢化合物（包括正构烷烃，烯烃和正构烷基酯）是含量最高的组分，总收率在总离子强度（TII）的7.4％至15.7％之间。当真菌细胞在脂肪酸和PDB上生长时，细胞外链烷烃的C链长度范围为n-C19至n-C34，烯烃n-C30：1- nC40：1和长链烷基酯（C41-C60：1） 。根据Py-FIMS分析，在PDB的氯仿提取物中未观察到烷烃，烯烃和烷基酯。后者表明所研究的混合真菌物种在生长过程中催化脂肪酸向碳氢化合物的转化。;通过Iatroscan TLC-FID分析了混合物种培养产生的不同脂质的化学分析，从而鉴定出15种不同的脂质类别。当混合物种培养在指数生长期在PDB + UDA上生长时，观察到最高的总细胞内脂质含量为3.5 mg / ml。游离脂肪酸是在指数生长期和静止生长期的细胞内和细胞外产生的最主要的脂质类别。它们占产生的脂质总量的80％以上。通过GC-FID分析对细胞内和细胞外饱和和不饱和脂肪酸的分析表明，在55种被分析的脂肪酸中，所有底物上最主要的脂肪酸为棕榈酸（C16），硬脂酸（C18），油酸（C18： 1）和亚油酸（C18：2），约占所产生的总游离脂肪酸（FFA）的70％。单不饱和脂肪酸和多不饱和脂肪酸的浓度相似，其含量为总FFA（0.007至0.57 mg / ml）的23％至66％，饱和脂肪酸的浓度为FFA的33％至75％。总FFA（0.01至1.97 mg / ml）取决于底物类型和生长期。

著录项

  • 作者

    Chahal, Amarpreet.;

  • 作者单位

    Carleton University (Canada).;

  • 授予单位 Carleton University (Canada).;
  • 学科 Biology Microbiology.;Chemistry Biochemistry.;Chemistry Analytical.
  • 学位 M.Sc.
  • 年度 2013
  • 页码 178 p.
  • 总页数 178
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号