• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Identification of RFLP markers associated with cytoplasmic male sterility and the survey of cytoplasmic genome variation in Capsicum annuum L.
【24h】

Identification of RFLP markers associated with cytoplasmic male sterility and the survey of cytoplasmic genome variation in Capsicum annuum L.

机译：与辣椒胞质雄性不育相关的RFLP标记的鉴定和胞质基因组变异的调查

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In chile (Capsicum annuum L.), the available knowledge on the behavior of cytoplasmic male sterility (CMS) is relatively limited. Thus, molecular markers that would assist the establishment of CMS based hybrid seed production are not available for breeding purposes. To overcome this gap, and aid the currently employed backcrossing, and testcrossing breeding techniques, identification of molecular markers was targeted in this study.;Similarly, to other plant species, restriction fragment length polymorphism (RFLP) provided an effective tool to identify polymorphic mitochondrial regions that can distinguish individuals regarding their cytoplasm. The nine mitochondrial probe and five restriction enzyme combinations could identify three ( atp9, atp6 and MOC1) polymorphic mitochondrial regions between sterile (S) and normal (N) cytoplasm types that can serve as molecular marker for cytoplasmic comparisons in Capsicum.;The "newly" identified MOC1 (Lycopersicum pennellii) mitochondrial probe was 100% accurate in identifying sterile (S) and normal (N) cytoplasm genotypes through backcrossing, and also aided the study of the CMS related mitochondrial rearrangements in Capsicum. Southern hybridizations with MOC1 could not reveal divergence among the various S-cytoplasm sources of Capsicum annuum indicating that a similar mutation exists for all the tested sterile Capsicum cytoplasm sources. However, some variation was observed within the normal cytoplasm-types.;Based on the MOC1 (Lycopersicum pennellii) sequence information, PCR primers were designed to span the coxII mitochondrial gene region in Capsicum. Study of the MOC1-RFLP marker indicated mitochondrial region showed that the CMS associated rearrangements do not involve the coding region of coxII, but entails its 3' UTR. Besides, homologous region to the MOC1 (Lycopersicum pennellii ) encoding region in chile cannot be amplified. Nevertheless, to increase the MOC1's commercial feasibility (convert into a PCR marker), the Capsicum coxII 3'-flanking region needs to be further studied, so that specific primers can be designed directly from the rearranged mitochondrial region. Because the association of the MOC1 detected polymorphism at DNA level and the expression of sterility is still unknown, the MOC1-RFLP marker remains to be an effective indirect marker for comparing of male sterile and male fertile cytoplasms in Capsicum.
机译：在智利（ Capsicum annuum L。），关于细胞质雄性不育（CMS）行为的可用知识相对有限。因此，不能用于建立基于CMS的杂种种子生产的分子标记不能用于育种目的。为了克服这一差距，并帮助目前使用的回交和testcrossing育种技术，本研究的目标是鉴定分子标记。类似地，对于其他植物，限制性片段长度多态性（RFLP）提供了一种有效的工具来鉴定线粒体的多态性可以区分个人的细胞质区域。九种线粒体探针和五种限制性内切酶组合可以识别无菌（S）和正常（N）细胞质类型之间的三个（ atp9 ， atp6 和MOC1）多态性线粒体区域。用作辣椒中细胞质比较的分子标记。“新近”鉴定的MOC1（ Lycopersicum pennellii ）线粒体探针可100％准确地鉴定出无菌（S）和正常（ N）通过回交的细胞质基因型，也有助于研究辣椒中CMS相关的线粒体重排。与MOC1的Southern杂交不能显示 Capsicum annuum 的各种S细胞质来源之间的差异，表明所有测试的 Capsicum 细胞质来源都存在相似的突变。然而，在正常细胞质类型中观察到一些变化。；基于MOC1（ Lycopersicum pennellii ）序列信息，设计PCR引物以跨越 coxII 线粒体基因区域。在 Capsicum 中。对MOC1-RFLP标记的研究表明，线粒体区域显示CMS相关的重排不涉及 coxII 的编码区，但需要其3'UTR。此外，智利的MOC1（ Lycopersicum pennellii ）编码区的同源区域无法扩增。然而，为了增加MOC1的商业可行性（转换为PCR标记），需要进一步研究 capsicum coxII 3'侧翼区域，以便可以直接从重排的线粒体区域设计特异性引物。 。由于MOC1在DNA水平检测到的多态性与不育表达之间的关联仍然未知，因此MOC1-RFLP标记仍然是比较 Capsicum 中雄性不育和雄性可育细胞质的有效间接标记。 。

著录项

  • 作者

    Pakozdi, Katalin Monika.;

  • 作者单位

    New Mexico State University.;

  • 授予单位 New Mexico State University.;
  • 学科 Agriculture Agronomy.;Biology Botany.;Biology Genetics.;Agriculture Plant Culture.;Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 95 p.
  • 总页数 95
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号