• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Caracterisation genetique et etude de l'antibioresistance d'isolats de Campylobacter retrouves chez le porc, la volaille et l'humain.
【24h】

Caracterisation genetique et etude de l'antibioresistance d'isolats de Campylobacter retrouves chez le porc, la volaille et l'humain.

机译：在猪，家禽和人类中发现的弯曲杆菌分离株的遗传特征和对抗生素耐药性的研究。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Campylobacter is recognized as the main cause of bacterial diarrhea in humans worldwide. Most infections in humans are caused by C. jejuni and C. coli and remain sporadic. The transmission occurs most of the time by contaminated water, unpasteurized milk or contaminated food. Meat from poultry, pigs and cattle is considered among the most frequent vector of transmission for the disease. The high rate of Campylobacter recovered from these animals increases the risk of contamination of the carcasses following the slaughtering process. Improperly handled or cooked food may thus result in the development of Campylobacter infections. Little is known about the risk of contamination for humans associated with the consumption of meat products from swine. The analysis and the characterization of this risk were at the origin of the current study.; Intestinal contents from 850 healthy pigs, collected at the slaughterhouse, and 278 samples of feces from patients with diarrhea were analyzed. Among the Campylobacter recovered, a sub-set of 100 isolates from swine and all 24 isolates from humans were characterized genotypically and phenotypically by PFGE and in vitro toxins assays.; A high genetic diversity, near 80%, was observed among the two Campylobacter populations. Genetic diversity was also observed among isolates originating from the same animal. However, no genetic link between isolates from human and from swine was observed in this study. The phenotypic analysis of toxins production by Campylobacter demonstrated that isolates recovered in this study had weak cytotoxicity properties in vitro.; The recent increase in resistance to antimicrobial agents among Campylobacter represents another concern for human health. Some bacterial strains from meat could represent a potential reservoir of resistant isolates transmissible to humans. Also, the wide use of antimicrobial agents at the farm level could select for resistant isolates and promote the persistence of those strains.; In the current study, the great majority of Campylobacter isolates from swine, poultry and human were resistant to tetracycline. Also, our results suggest that some resistant isolates could express another genetic determinant than tetO for resistance to tetracycline. Resistance to fluoroquinolone in Campylobacter, an emerging problem in public health, was observed in this study. Among the C. jejuni from humans, 8.7% were resistant to ciprofloxacin.; An analysis on the impact of the use of antimicrobial agents was done among 27 swine herds, focusing on farms using or not antimicrobial agents of tetracycline or fluoroquinolone type. The analysis of Campylobacter isolates from those farms showed that there was no significant difference between rates of resistance in bacteria among herds with or without use of tetracycline or enrofloxacin.
机译：弯曲杆菌被认为是全世界人类细菌性腹泻的主要原因。人类大多数感染是由空肠弯曲杆菌和大肠杆菌引起的，并且仍然是零星的。传播大部分时间是由受污染的水，未经巴氏消毒的牛奶或受污染的食物引起的。家禽，猪和牛的肉被认为是该病最常见的传播媒介。从这些动物中回收的弯曲杆菌的高比率增加了屠宰后屠体受到污染的风险。处理或烹饪食物不当可能会导致弯曲杆菌感染。关于从猪食用肉制品对人类造成污染的风险知之甚少。这种风险的分析和表征是本研究的起源。分析了屠宰场收集的850头健康猪的肠内容物，以及腹泻患者的278份粪便样本。在回收的弯曲杆菌中，通过PFGE和体外毒素分析对猪的100个分离株和人的所有24个分离株进行了基因型和表型鉴定。在两个弯曲杆菌种群中观察到很高的遗传多样性，接近80％。在同一动物的分离株中也观察到遗传多样性。但是，在这项研究中未观察到人类和猪的分离株之间的遗传联系。弯曲杆菌产生毒素的表型分析表明，在这项研究中回收的分离株在体外具有弱的细胞毒性。弯曲杆菌中对抗菌剂的抗药性最近增加代表了对人类健康的另一个关注。肉类中的某些细菌菌株可能代表了可能传播给人类的抗性分离株的潜在库。同样，在农场一级广泛使用抗微生物剂可以选择抗药性菌株并促进这些菌株的持久性。在当前的研究中，来自猪，家禽和人的大多数弯曲杆菌分离株对四环素具有抗性。同样，我们的结果表明，某些抗药性菌株可能表达出比tetO更强的四环素抗性遗传决定因子。在这项研究中观察到了弯曲杆菌对氟喹诺酮类药物的耐药性，弯曲杆菌是公共卫生中一个新出现的问题。在来自人的空肠弯曲杆菌中，有8.7％对环丙沙星耐药。在27个猪群中对使用抗菌剂的影响进行了分析，重点是使用或不使用四环素或氟喹诺酮类抗菌剂的农场。对来自这些农场的弯曲杆菌分离株的分析表明，在有或没有使用四环素或恩诺沙星的畜群中，细菌的耐药率之间没有显着差异。

著录项

  • 作者

    Guevremont, Evelyne.;

  • 作者单位

    Universite de Montreal (Canada).;

  • 授予单位 Universite de Montreal (Canada).;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 158 p.
  • 总页数 158
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号