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首页> 外文学位 >Effects of Heterodera glycines population densities on Fusarium solani f. sp. glycines colonization of soybean roots and development of sudden death syndrome.
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Effects of Heterodera glycines population densities on Fusarium solani f. sp. glycines colonization of soybean roots and development of sudden death syndrome.

机译：异杂藻甘氨酸种群密度对茄镰刀菌的影响。 sp。大豆根部甘氨酸的定殖和猝死综合征的发展。

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摘要

Sudden death syndrome (SDS), caused by Fusarium solani f. sp. glycines (Fsg), is an important disease of soybean in the U.S. Soybean cyst nematode, Heterodera glycines, has been identified in every U.S. region where soybean is grown and exacerbated SDS symptoms in previous greenhouse experiments, but the interaction has not been defined in the field. Experiments were conducted in six field environments and two greenhouse experiments with four Essex x Forrest near-isogenic soybean lines (NILs) with four combinations of resistance and susceptibility to both pathogens to investigate the effects of H. glycines on SDS development and root colonization by the fungus. Fsg colonization was estimated in colony-forming units (CFU) with a traditional plating bioassay on a semi-selective medium. High variability in the field data made it difficult to draw conclusions from these experiments. Substantial disease only developed in one environment on the SSDSRSCN NIL. Variability in the data was less from the greenhouse experiments, but still high for CFU. Area under the SDS progress curve did not differ between NILs. However, disease severity increased linearly with increasing nematode densities at planting across NILs that were infected with a low level of Fsg. The results from these experiments suggest that H. glycines worsens SDS symptoms under some conditions. Cultivars with resistance to both pathogens should be planted in fields with a history of both pathogens.; Estimation of root colonization by Fsg with the plating bioassay was difficult and the results were often unsatisfactory in these experiments. Therefore, an alternative method was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in 14.5% of the samples with the plating bioassay. The plating bioassay required at least 10 days longer to complete and the results had higher variability than those obtained with QPCR. Overall, QPCR had many advantages over the plating bioassay and has the potential for several more practical applications.
机译：枯萎镰刀菌引起的猝死综合征（SDS）f。 sp。甘氨酸（Fsg）是美国大豆中的一种重要疾病。大豆囊肿线虫Heterodera甘氨酸已在以前的温室试验中在每个有大豆生长并加剧SDS症状的美国地区发现，但相互作用尚未在大豆中定义。领域。在六个田间环境中进行了实验，并在两个温室实验中使用了四个Essex x Forrest近等基因大豆系（NIL），对这两种病原体具有四种抗药性和敏感性，以研究甘氨酸对甘草素对SDS发育和根系定植的影响。菌。在半选择培养基上，采用传统的平板生物测定法，以菌落形成单位（CFU）估算Fsg的定殖。现场数据的高度可变性使得很难从这些实验中得出结论。仅在SSDSRSCN NIL上的一种环境中才发生重大疾病。温室实验中数据的变异性较小，但CFU仍较高。在NIL之间，SDS进度曲线下的面积没有差异。但是，在严重感染Fsg水平较低的NIL上种植时，病害的严重程度随线虫密度的增加而线性增加。这些实验的结果表明，在某些条件下，甘氨酸链球菌会使SDS症状恶化。对两种病原体均具有抗性的品种应种植在有两种病原体病史的田间。用平板生物测定法估计通过Fsg进行的根定殖很困难，并且在这些实验中结果通常不令人满意。因此，探索了一种替代方法，即使用实时定量PCR（QPCR）进行相对定量，以估计大豆根部的真菌定植。 QPCR在检测真菌方面比电镀生物分析更为灵敏：通过QPCR在372个根样品中的371个中检测到Fsg DNA，而在电镀生物分析中仅在14.5％的样品中检测到CFU。平板生物测定至少需要10天以上的时间才能完成，结果的变异性比QPCR更高。总体而言，QPCR与电镀生物测定法相比具有许多优势，并且有可能用于更多实际应用中。

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  • 作者

    Jackson, Tamra A.;

  • 作者单位

    University of Illinois at Urbana-Champaign.;

  • 授予单位 University of Illinois at Urbana-Champaign.;
  • 学科 Agriculture Plant Pathology.; Agriculture Agronomy.; Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 128 p.
  • 总页数 128
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;农学（农艺学）;微生物学;
  • 关键词

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