首页> 外文学位 >Documentation, characterization, and proposed mechanism of diquat resistance in Landoltia punctata (G. Meyer) D. H. Les and D. J. Crawford.
【24h】

Documentation, characterization, and proposed mechanism of diquat resistance in Landoltia punctata (G. Meyer) D. H. Les and D. J. Crawford.

机译:Landoltia punctata(G.Meyer)D.H.Les和D.J.Crawford的对百草枯抗性的文献资料,特征和拟议的机制

获取原文
获取原文并翻译 | 示例

摘要

Trials conducted with an accession of landoltia [Landoltia punctata (G. Meyer) D.H. Les and D.J. Crawford] collected from a canal in Lake County, FL, showed developed resistance to diquat [resistance factor (Rf) of 50], and cross resistance to paraquat (Rf 29). Diquat resistance in landoltia was independent of photosynthesis, and the response to diquat was delayed compared to that of a biotype with no history of herbicide treatment (S). Less diquat was likely transported into the protoplast. Whole plant uptake of 14C-diquat was also inhibited and reduced in resistant (R) compared to the S biotype in both light and dark conditions, although differences in uptake could not explain the 50-fold resistance. Data indicate that less diquat was absorbed by the R biotype in light treatments, although the percentage difference between the two biotypes was minimal (20%) and not consistent across all experiments, possibly due to differential diquat accumulation in the cytoplasm. Copper applied in combination with diquat overcame the diquat resistance in landoltia, and increased susceptibility of the R biotype to that of the S biotype. Copper probably increased permeability of the cell membrane to diquat by causing the formation of non-specific ion channels, as electrolyte leakage from copper exposure alone was rapid (1 hr). Therefore, copper may alter the transport mechanism for diquat across the plasmalemma or open a secondary site for transport. Copper reportedly reacts with calcium (Ca2+) channels in the plant cell membrane creating non-specific ion channels. Diquat activity was reduced on the S biotype of landoltia preconditioned with the Ca2+ channel blocker lanthanum chloride (Rf = 86). This reduction of activity suggests that lanthanum was effective at inhibiting transport of diquat to the site of action, although lanthanum could also alter membrane permeability and evolution of radical oxygen. The relationship among copper, diquat, calcium channels, and lanthanum warrants further study as it relates to diquat transport and potential resistance mechanisms. These studies have documented the first aquatic plant to become resistant to the bipyridylium herbicides, and suggest that the resistance mechanism is related to reduced herbicide transport across cell membranes.
机译:加入兰多菌[Landoltia punctata(G.Meyer)D.H. Les和D.J.从佛罗里达州莱克县的一条运河收集的克劳福德[Crawford]对敌草快具有抗药性[抵抗力(Rf)为50],对百草枯的交叉抗药性(Rf 29)。与没有除草剂处理史的生物型相比,兰地鼠中对敌草快的抗性与光合作用无关,对敌草快的响应也有所延迟。可能更少的敌草快被运输到原生质体中。与S生物型相比,在明亮和黑暗条件下,全植物对14C-敌草快的吸收均受到抑制并降低了抗性(R),尽管吸收差异不能解释50倍抗性。数据表明,在光处理中,R生物型吸收的敌草快较少,尽管两种生物型之间的百分数差异很小(<20%),并且在所有实验中不一致,这可能是由于敌草快在细胞质中的积累所致。与敌草快联用的铜克服了兰地鼠对敌草快的抵抗力,使R生物型对S生物型的敏感性增加。铜可能会引起非特异性离子通道的形成,从而增加细胞膜对敌草快的渗透性,因为单独铜暴露引起的电解质泄漏很快(<1小时)。因此,铜可能会改变敌草快穿过质膜的转运机制,也可能会打开次要部位进行转运。据报道,铜与植物细胞膜中的钙(Ca2 +)通道发生反应,形成非特异性离子通道。用Ca2 +通道阻滞剂氯化镧(Rf = 86)预处理的兰地菌S生物型的敌草快活性降低。活性的降低表明,镧可以有效地抑制敌草快向作用部位的转运,尽管镧也可以改变膜的渗透性和自由基氧的释放。铜,敌草快,钙通道和镧之间的关系值得进一步研究,因为它与敌草快的运输和潜在的耐药机制有关。这些研究记录了第一种对双吡啶鎓除草剂具有抗性的水生植物,并表明该抗性机理与减少除草剂跨细胞膜的运输有关。

著录项

  • 作者

    Koschnick, Tyler J.;

  • 作者单位

    University of Florida.;

  • 授予单位 University of Florida.;
  • 学科 Agriculture Agronomy.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 123 p.
  • 总页数 123
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农学(农艺学);植物学;
  • 关键词

  • 入库时间 2022-08-17 11:42:23

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号