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Stress-induced germination vigor and its translation to seedling vigor in Beta vulgaris L.

机译：寻常型甜菜的胁迫诱导萌发活力及其向幼苗活力的转化

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摘要

Beta vulgaris L., sugarbeet, is an important plant for sucrose production in the U.S. and worldwide. One limitation for sugarbeet production is poor germination. Breeding for improved germination and seedling vigor has been unsuccessful due to low heritability of traits controlling germination vigor, few molecular markers and high environmental variability. Germination and seedling responses to stress in two beet varieties with differing vigor were studied in hydrogen peroxide and water. Differences in water absorption and internal hydrogen peroxide concentrations were observed between varieties. Gene expression changes were identified qualitatively (343 genes) and quantitatively (48 genes) during germination. In both varieties, germination was characterized by a rapid uptake of water and rapid gene activation within the first 24 h of imbibition. Upregulation of putative LTP4 (lipid transfer protein), MPK4 (MAP Kinase 4), BRI1 (Brassinosteroid 1), and MKK9 (MAPK Kinase 9) was associated with response to H2O2. The 48 genes, tested for expression in germination vigor, were evaluated for their ability to predict vigor in 3-week old seedlings of the same two varieties. Gene expression of seedlings treated with H 2O, Aphanomyces sp., or Rhizopus sp. had different quantitative and temporal expression patterns depending on the variety and treatment. BRI1 and CAF1 (CCR4-NOT Associated Factor) were upregulated in both varieties in response to pathogen treatments. Of the 48 genes quantitatively tested between seeds and seedlings, varieties and the stress treatments, only BRI1, LTP4 and MKK9 were consistently upregulated. These genes may be useful as molecular markers for breeding efforts to enhance seedling vigor.
机译：甜菜Beta vulgaris L.是美国和世界范围内生产蔗糖的重要植物。甜菜生产的限制之一是发芽差。由于控制发芽活力的性状的遗传力低，分子标记物少和环境变异性高，因此未能提高育种和幼苗活力的育种未能成功。在过氧化氢和水中研究了两个活力不同的甜菜品种的发芽和幼苗对胁迫的响应。品种之间观察到吸水率和内部过氧化氢浓度的差异。在发芽过程中定性地（343个基因）和定量地（48个基因）识别基因表达变化。在这两个品种中，发芽的特征都是在吸水的最初24小时内迅速吸收水分并快速激活基因。假定的LTP4（脂质转移蛋白），MPK4（MAP激酶4），BRI1（油菜素类固醇1）和MKK9（MAPK激酶9）的上调与对H2O2的反应有关。测试了48个基因在萌发活力中的表达，评估了它们在相同两个品种的3周龄幼苗中预测活力的能力。 H 2O，Aphanomyces sp。或Rhizopus sp。处理的幼苗的基因表达。根据品种和处理方式的不同，其定量和时间表达模式也不同。响应病原体处理，两个品种中的BRI1和CAF1（CCR4-NOT相关因子）均被上调。在种子和幼苗，品种和胁迫处理之间定量测试的48个基因中，只有BRI1，LTP4和MKK9一直被上调。这些基因可用作分子标记，用于育种以增强幼苗活力。

著录项

  • 作者

    Naegele, Rachel P.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Plant Culture.;Biology Genetics.;Biology Molecular.
  • 学位 M.S.
  • 年度 2010
  • 页码 145 p.
  • 总页数 145
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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